NEXTFLEX Myeloid Amplicon Panel2018-08-31T19:08:12+00:00

VALIDATED PANEL FOR DETECTION OF 105 SELECTED EXONS, FROM 21 MYELOID RELATED GENES, WITH LIBRARY PREP REAGENTS & BARCODES FOR ILLUMINA® SEQUENCING

NEXTFLEX® Myeloid Amplicon Panel

  • 100% coverage of 105 selected exons from 21 different genes and flanking intron-exon boundaries
  • 95% uniformity of amplicons at 0.2x mean coverage; 99% of amplicons detected at 0.1X mean coverage
  • 384 unique barcodes available upon request
  • Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and barcodes
  • Low input – Only 40 ng DNA isolated from fresh or frozen samples required

  • NOVA-4260-01

       $704

    8 BACODES | 8 RXNS

  • NOVA-4260-02

       $4,032

    48 BACODES | 48 RXNS
Download Manual
Covered Regions
For research use only. Not for use in diagnostic procedures.

Coverage of the selected Genes for Illumina® Sequencing Platforms

The NEXTFLEX® Myeloid Amplicon Panel allows you to quickly target and sequence 105 selected exons from 21 different genes loci. This kit contains primer pairs and reagents needed to amplify these coding regions from DNA isolated from fresh or frozen material, and prepare libraries for Illumina® sequencing.

The NEXTFLEX® Myeloid Amplicon Panel has the capability of multiplexing up to 4 samples at 2000x coverage for detection of somatic mutations on a single Illumina® 2×300 MiSeq® lane.


A Complete Solution for Targeted Sequencing

The NEXTFLEX® Myeloid Amplicon Panel is a complete, validated solution that targets 105 selected exons from 21 different genes loci using 270 PCR primer pairs. This kit requires only 40 ng of DNA isolated from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.

GeneCovered Region
ASXL1Exon 12
CALRExon 9
CEBPAAll CDS*
CSF3RExon 14, 17
DNMT3AAll CDS*
EZH2All CDS*
FLT3Exon 14-15, 20
IDH1Exon 4
IDH2Exon 4
JAK2Exon 12, 14
JAK3Exon 13
KITExon 2, 8-11, 13, 17
MPLExon 10
NPM1Exon 11
RUNX1All CDS*
SETBP1Exon 4 (hotspot)
SF3B1Exon 13-16
SRSF2Exon 2
TET2Exon 3-11
TP53Exon 2-11
U2AF1Exon 2, 5, 6

* CDS = Coding Sequences

KIT CONTENTS

  • NEXTFLEX® Myeloid Amplicon Primer Mix 1 and 2
  • NEXTFLEX®  Myeloid Amplicon Primer Mix 3 and 4
  • NEXTFLEX®  Hot Start PCR I Master Mix
  • NEXTFLEX®  Hot Start PCR I Master Mix (A)
  • NEXTFLEX® PCR Enhancer 1
  • NEXTFLEX® PCR Enhancer 2
  • NEXTFLEX® PCR Enhancer 5
  • NEXTFLEX® Stop Solution
  • NEXTFLEX®  Ligation Mix
  • NEXTFLEX®  Amplicon DNA Adapter
  • NEXTFLEX®  PCR II Barcoded Primer Mix
  • NEXTFLEX® PCR II Master Mix
  • Nuclease-free Water
  • Resuspension Buffer
  • NEXTFLEX® Cleanup Beads

REQUIRED MATERIALS NOT PROVIDED

  • 40 – 200 ng of extracted genomic DNA (four 10 – 50 ng aliquots in up to 19.8 μL nuclease-free water each)
  • Ethanol 80% (room temperature)
  • 96 well PCR Plate Non-skirted (Phenix Research, Cat # MPS-499) or similar
  • 96 well Library Storage and Pooling Plate (Fisher Scientific®, Cat # AB-0765) or similar
  • Adhesive PCR Plate Seal (Bio-Rad®, Cat # MSB1001)
  • Magnetic Stand -96 (Thermo Fisher® Scientific, Cat # AM10027) or similar
  • Thermocycler
  • 2, 10, 20, 200 and 1000 μL pipettes / multichannel pipettes
  • Nuclease-free barrier pipette tips
  • Microcentrifuge
  • 1.5 mL nuclease-free microcentrifuge tubes
  • Vortex

NEXTFLEX Myeloid Amplicon Panel Flow Chart

The NEXTFLEX®  Myeloid Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

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