NEXTFLEX BRCA1 and BRCA2 Amplicon Panel for FFPE2018-09-01T02:00:48+00:00

VALIDATED BRCA1/2 PANEL FOR USE WITH DNA ISOLATED FROM FFPE SAMPLES FOR ILLUMINA® SEQUENCING

NEXTFLEX® BRCA1 & BRCA2 Amplicon Panel for FFPE Illumina® Sequencing

  • Complete BRCA1 and BRCA2 coverage
  • 100% uniformity at ≥0.1x mean coverage, 97% uniformity at ≥0.2x mean coverage of all targeted coding exons and exon-intron boundaries, validated on DNA isolated from FFPE samples
  • 384 barcodes available upon request
  • Complete solution for targeted sequencing including gene specific primers, PCR Master Mix, clean-up beads, and barcodes
  • Low input – Only 40 ng DNA isolated from FFPE samples required

  • NOVA-4222-01

       $696

    8 BACODES | 8 RXNS

  • NOVA-4222-02

       $3,870

    48 BACODES | 48 RXNS
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Covered Regions
For research use only. Not for use in diagnostic procedures.

Complete BRCA1 and BRCA2 Coverage for Illumina® Sequencing of FFPE DNA

The NEXTFLEX® BRCA1 & BRCA2 Amplicon Panel for FFPE allows you to quickly target and sequence all coding exons of the BRCA1 and BRCA2 loci. This kit contains primer pairs and reagents needed to amplify the coding regions of BRCA1 and BRCA2 from DNA isolated from FFPE material and prepare libraries for Illumina® sequencing.

A Complete Solution for Targeted BRCA1/2 Sequencing

The NEXTFLEX® BRCA1 & BRCA2 Amplicon Panel for FFPE is a complete solution, validated with DNA isolated from FFPE samples, that targets all coding exons in BRCA1 and BRCA2 loci using 261 PCR primer pairs. This kit requires only 40 ng of DNA isolated from FFPE samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.

KIT CONTENTS

  • NEXTFLEX® BRCA Amplicon FFPE Primer Mix 1 – 4
  • NEXTFLEX® Hot Start PCR I Master Mix
  • NEXTFLEX® Stop Solution
  • NEXTFLEX® End Repair Buffer Mix
  • NEXTFLEX® End Repair Enzyme Mix
  • NEXTFLEX® Ligation Mix
  • NEXTFLEX® Amplicon DNA Adapter
  • NEXTFLEX® PCR II Barcoded Primer Mix 1-8
  • NEXTFLEX® PCR II Master Mix
  • Nuclease-free Water
  • Resuspension Buffer
  • NEXTFLEX® Cleanup Beads

REQUIRED MATERIALS NOT PROVIDED

  • 40 – 200 ng of DNA isolated from FFPE samples (four 10 – 50 ng aliquots in up to 20.4 µL nuclease-free water each)
  • Ethanol 80% (room temperature)
  • 96 well PCR Plate Non-skirted (Phenix Research, Cat # MPS-499) or similar
  • 96 well Library Storage and Pooling Plate (Fisher Scientific, Cat # AB-0765) or similar
  • Adhesive PCR Plate Seal (BioRad, Cat # MSB1001)
  • Magnetic Stand -96 (Ambion, Cat # AM10027) or similar
  • Thermocycler
  • 2, 10, 20, 200 and 1000 µL pipettes / multichannel pipettes
  • Nuclease-free barrier pipette tips
  • Microcentrifuge
  • 1.5 mL nuclease-free microcentrifuge tubes
  • Vortex

NEXTFLEX BRCA1/2 FFPPE Flow Chart

The NEXTFLEX®  BRCA1 & BRCA2 Amplicon Panel for FFPE contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from FFPE-fixed samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

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