NEXTFLEX DNA Barcode Blockers2018-09-06T15:06:03+00:00

FOR USE WITH NIMBLEGEN® SEQCAP® EZ LIBRARY OR AGILENT® SURESELECTXT2® TARGET CAPTURE KITS & ILLUMINA® SEQUENCING

NEXTFLEX® DNA Barcode Blockers

  • 96 barcode blockers available
  • Index-specific blocking oligos result in a higher % of on-target reads
  • Compatible with NEXTFLEX® DNA Barcodes and NEXTFLEX-96 DNA Barcodes
  • Validated in combination with Roche NimbleGen® SeqCap® EZ library and Agilent® SureSelectXT2® target capture kits for Illumina® sequencing
  • Simplifies and reduces the cost of target capture
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For research use only. Not for use in diagnostic procedures.

  • NOVA-514131

       $904

    UP TO 48 SEQCAP® RXNS
    32 SURESELECT® RXNS

  • NOVA-514132

       $1,507

    UP TO 96 SEQCAP® RXNS
    64 SURESELECT® RXNS

  • NOVA-514133

       $2,939

    UP TO 192 SEQCAP® RXNS
    128 SURESELECT® RXNS

  • NOVA-514136

       $5,680

    UP TO 384 SEQCAP® RXNS
    256 SURESELECT® RXNS

  • NOVA-514134

       $11,361

    UP TO 192 SEQCAP® RXNS
    128 SURESELECT® RXNS

One Set of Blockers, Two Solutions

The NEXTFLEX® DNA Barcode Blockers are designed for use with the NEXTFLEX® DNA Barcodes and NEXTFLEX-96 DNA Barcodes. Both the barcodes and blockers are compatible with Roche NimbleGen® SeqCap® EZ library and Agilent® SureSelectXT2 target capture systems. Other solution-based hybridization systems are also compatible, yielding varying numbers of reactions per kit. The NEXTFLEX® DNA Barcode Blockers are available in sets of 6, 12, 24, 48 or 96 blocking oligos, and are designed to prevent concatemerization of non-complimentary library fragments during solution-based hybridization assays. Alternatively, complete library preparation, barcode and blocker solutions are available. The NEXTFLEX® Rapid Pre-Capture Combo Kit includes NEXTFLEX® DNA library prep, NEXTFLEX® DNA Barcodes for multiplexing, and the NEXTFLEX® Barcode Blockers. The NEXTFLEX® Pre- and Post- Capture Combo Kit (Compatible with Agilent® SureSelectXT2®) includes NEXTFLEX® Rapid DNA library prep reagents, NEXTFLEX® DNA Barcodes, and the NEXTFLEX® Barcode Blockers.

The NEXTFLEX-96 Barcode Blockers are the Most Affordable Blocking Solution for SeqCap® Target Capture

The NEXTFLEX-96 Barcode Blockers allow for multiplexing up to 96 samples in 192 target capture reactions, for a total of 18,432 individual libraries per kit (96 libraries x 192 target capture reactions = 18,432 samples processed). This translates to a cost of only $0.58 per individual library.

Whitepapers are available demonstrating the efficacy of the NEXTFLEX® DNA Barcode Blockers for improved target capture specificity, for the construction of high quality libraries using the NimbleGen® SeqCap® EZ Library and Agilent® SureSelectXT2® target capture systems.

KIT CONTENTS

  • NEXTFLEX® HE Universal Oligo 1
  • NEXTFLEX® INV-HE Index Oligos

WHITE PAPERS

  • An Integrated Solution to Simplify Library Preparation and Multiplexing for NimbleGen Sequence Capture
  • Improved Library Prep Offers Higher Percentage of On-target Reads and Better Coverage for SureSelectXT2 Target Capture
The NEXTFLEX® DNA Barcode Blockers contain 6, 12, 24, 48 or 96 oligo blockers designed to be used in conjunction with NEXTFLEX® DNA Barcodes. These kits ship on dry ice.
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