Predesigned AIR Adenylated Linkers2018-08-31T19:58:28+00:00

FOR CLONING miRNAS & OTHER SMALL RNAS FROM PRIMARY RNA SOURCES

Predesigned AIR Adenylated Linkers

  • Ideal for next-generation sequencing, miRNA cloning, and small RNA sequencing
  • 3’ end blocks prevent self-ligation
  • Pre-designed adapters are available with Ban-1, Ava-1, Sty-1, EcoR-1
  • Affordable alternative to other adapter suppliers
For research use only. Not for use in diagnostic procedures.

  • NOVA-510201

       $148

    SET A | 1 nm

  • NOVA-510205

       $504

    SET A | 5 nm

  • NOVA-510301

       $148

    SET B | 1 nm

  • NOVA-510305

       $504

    SET B | 5 nm

  • NOVA-510401

       $148

    SET C | 1 nm

  • NOVA-510405

       $504

    SET C | 5 nm

Adenylated Linkers for miRNA or Small RNA Cloning

Our AIR Adenylated Linkers are 5’adenylated oligos with 3’end blocks, which are ready for use in miRNA sequencing or small RNA cloning. AIR Adenylated Linkers are activated adenylated oligos that preclude the need for ATP in RNA Ligase 2 (RNL2) reactions. This allows for specific and clean ligations.

Restriction Site Sequence

AIR Adenylated Linker A contains the Ban-1 restriction site sequence: 
5’rAppCTGTAGGCACCATCAAT/3ddC/3’

AIR Adenylated Linker B contains the Ava-1 and Sty-1 restriction site sequence:
5’rAppCACTCGGGCACCAAGGA/3ddC/3’

AIR Adenylated Linker C contains the EcoR-1 restriction site sequence 
5’rAppTTTAACCGCGAATTCCAG/3ddC/3’

To request a quote for custom adenylated adapters contact us at Bioo.NGS@perkinelmer.com.

Available in 1 nm and 5 nm quantities and custom orders are also available.

Publication that Cites Using AIR™ Adenylated Linkers:

  • Verbruggen, S. Ribosome profiling, a useful tool in the search for micropeptides. MS Thesis. University of Gent, 2015.
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