Reducing Small RNA Ligation Bias – Small RNA Library Prep Solutions for Illumina® Sequencing
Small RNA sequencing is ideal for quantitative profiling of small RNAs (microRNAs, piRNAs and snoRNAs). It is used to analyze tissue specific miRNA expression patterns, disease associations and to discover previously uncharacterized miRNAs. However, ligation specific biases reduce the ability of small RNA-seq to accurately quantify microRNA. These biases are mitigated through the incorporation of adapters with degenerate bases during library preparation.
PerkinElmer’s line of NEXTFLEX® Small RNA Kits for Illumina® sequencing are ideal for the conversion of these small RNA transcripts into barcoded cDNA libraries for next-generation sequencing on Illumina® sequencing platforms. With the incorporation of randomized adapters to reduce bias and AIR™ ligase to increase ligation efficiency; the NEXTFLEX® Small RNA kit v3 offers better data quality and a greater proportion of productive of sequence reads than competitive small RNA library prep protocols.
- Small RNA-Seq Kit with Unique Dual Index Barcodes
- NEXTFLEX® Small RNA-seq v3 Automation Kit with UDIs – uses the same popular chemistry of the NEXTFLEX small RNA-seq kit v3, which incorporates technologies that reduce small RNA ligation bias, increase ligation efficiency, reduce adapter-dimer formation, and includes Unique Dual Indexes (UDIs) to mitigate index hopping for Illumina® sequencing on the MiSeq®, HiSeq® 2000/2500, and NovaSeq® platforms.
Small RNA Library Prep Kits
- NEXTFLEX® Small RNA-Seq Kit v3 – A small RNA library prep kit for Illumina® sequencing which provides randomized adapters for bias reduction along with gel-free or low-input protocols
Small RNA and mRNA Library Prep in a Single Workflow
- NEXTFLEX® Combo-Seq™ mRNA/miRNA Kit – Compatible with total RNA inputs, no poly(A) selection or rRNA depletion required
For research use only. Not for use in diagnostic procedures.