NEXTFLEX DNA-Seq Kit for Ion Torrent2018-09-07T20:44:31+00:00

DNA-SEQ LIBRARY PREP FOR ION S5, ION S5 XL, ION TORRENT PGM & ION PROTON SEQUENCERS

NEXTFLEX® DNA-Seq Kits for Ion Torrent Sequencers

  • Enhanced Adapter Ligation Technology offers larger number of unique sequencing reads
  • Flexible barcode options– Kits contain 8, 16, 32, or 64 unique barcodes
  • Simplified workflow with 96-well plate compatible volumes
  • Ideal for ChIP-seq and DNA-seq library construction for Ion Torrent sequencing
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For research use only. Not for use in diagnostic procedures.

  • NOVA-4001-01

       $181

    8 RXNS

  • NOVA-4001-02

       $860

    48 RXNS

  • NOVA-401001

       $401

    8 BARCODES | 48 RXNS

  • NOVA-401002

       $802

    16 BARCODES | 96 RXNS

  • NOVA-401003

       $1,605

    32 BARCODES | 192 RXNS

  • NOVA-401004

       $3,210

    64 BARCODES | 384 RXNS

Rapid Cell Free DNA Library Prep for Sequencing on Ion Torrent Platforms

The NEXTFLEX® DNA Sequencing Kit for Ion Torrent sequencing is designed to prepare genomic DNA-Seq or ChIP-Seq NGS libraries for sequencing using the Ion S5, Ion S5 XL, Ion Torrent PGM, and Ion Proton sequencing platforms. The NEXTFLEX® DNA Sequencing Kit features Enhanced Adapter Ligation Technology, resulting in library preps with a larger number of unique sequencing reads. This DNA-Seq library prep kit simplifies the Ion Torrent-based library prep workflow by using master mixed reagents and gel-based cleanup. In addition, the option of performing the reactions in a pre-prepared 96-well plate makes this kit more automation friendly than the other available versions.

Ion S5, Ion PGM and Ion Proton DNA-Seq Multiplexing Options

The NEXTFLEX® DNA Barcodes for Ion Torrent sequencing are adapters containing indexed sequences that offer an improved multiplexing workflow and flexible setup. This format enables multiplexing of up to 64 samples. The NEXTFLEX® DNA Barcodes are available in sets of 8, 16, 32, and 64 unique adapters. The ability to pool samples in an efficient way significantly decreases hands-on time while providing robust data quality.

KIT CONTENTS

  • NEXTFLEX® End Repair Buffer Mix
  • NEXTFLEX® End Repair Enzyme Mix
  • NEXTFLEX® Ligation Mix
  • NEXTFLEX® Adapter Mix (6.25 µM)
  • NEXTFLEX® Primer Mix (12.5 µM)
  • NEXTFLEX® 5X PCR Master Mix
  • 6X Loading Dye
  • MW Ladder Ready-to-Load 100 bp
  • Column Elution Buffer
  • Nuclease-free Water
  • Resuspension Buffer
  • DNA Binding Buffer
  • 5X DNA Wash Buffer
  • Clean-Up Spin Columns

REQUIRED MATERIALS NOT PROVIDED

  • 10 ng, 100 ng or 1 µg of fragmented genomic DNA in up to 40 µL nuclease-free water.
  • NEXTFLEX® DNA Barcodes Ion Torrent Compatible – 8 / 16 / 32 / 64 (Cat # 4010-01, 4010-02, 4010-03, 4010-04)
  • Ethanol 100% (room temperature)
  • Ethanol 80% (room temperature)
  • 96 well PCR Plate Non-skirted (Phenix Research, Cat # MPS-499) / or / similar
  • 96 well Library Storage and Pooling Plate (Fisher Scientific, Cat # AB-0765) / or / similar
  • Adhesive PCR Plate Seal (BioRad, Cat # MSB1001)
  • Agencourt AMPure XP 5 mL (Beckman Coulter Genomics, Cat # A63880)
  • Magnetic Stand -96 (Ambion, Cat # AM10027) / or / similar
  • Heat block
  • Thermocycler
  • 2, 10, 20, 200 and 1000 mL pipettes / multichannel pipettes
  • Nuclease-free barrier pipette tips
  • Microcentrifuge
  • 1.5 mL nuclease-free microcentrifuge tubes
  • Low melt agarose such as Low Gelling Temperature Agarose with a melt point of 65ºC (Boston Bioproducts, Cat # P-730) / or / E-gel system
  • 1X TAE buffer
  • Clean razor or scalpel
  • SYBR Gold (Invitrogen, Cat # S11494)
  • UV transilluminator or gel documentation instrument
  • Gel electrophoresis apparatus
  • Electrophoresis power supply
  • Vortex
The NEXTFLEX® DNA-Seq Library Prep Kits for Ion Torrent sequencing contain the reagents to prepare 8 or 48 genomic or ChIP DNA samples for Ion Torrent next-generation sequencing. The shelf life of all reagents is 12 months when stored properly at -20°C. Ships on dry ice.
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