NGS Library Prep Kits for Illumina® & Ion Torrent™ Sequencing
High-quality library preparation is a critical step in next-generation sequencing (NGS). PerkinElmer provides a complete portfolio of NGS library preparation kits and barcodes designed to increase the sensitivity, flexibility, and speed of library prep for the Illumina® and Ion Torrent™ sequencing platforms. We have focused our attention on increasing enzymatic efficiency in our next generation sequencing library prep kits to cater to the customer’s need for a cost effective solution to achieve robust, reproducible, high-quality sequencing results every time.
Completely automated versions of our library prep kits are available for high throughput needs while manual versions cater to the needs of lower throughput users. To meet the demands of high-throughput customers, NEXTFLEX® reagents can also be supplied in bulk quantities and custom formats.
///// Our library prep kits are optimized to simplify the following applications:
NGS Adapters | Barcoded adapters for all of your NGS multiplexing needs
Small RNA-Seq | Gene silencing and post-transcriptional regulation of gene expression can be investigated by small RNA-seq, which allows for the profiling of all of the small RNA and miRNA present in samples
DNA-Seq | Whole genome sequencing for the identifications of the entire genome sequence of a given individual or organism
Targeted Sequencing | Targeted resequencing for a highly targeted approach that analyzes specific genomic regions such as the exome using hybrid-capture solutions
RNA-Seq | Profiling for the analysis of the expression level of RNAs in a given cell or population of cells
Preimplantation Genetic Testing | Aneuploidy detection of preimplantation embryos
RANGE OF LIBRARY PREPARATION KITS
Rapid DNA Library Prep for Sequencing on Illumina® Platforms
The NEXTFLEX® Rapid XP DNA-Seq kit combines enzymatic fragmentation with end-repair and A-tailing in one reaction to create a highly efficient first step in library generation, which is followed by ligation and PCR (optional for PCR-free workflows). This effective one-tube workflow produces DNA-seq libraries with consistent library size, high yield, low GC-bias, and high coverage. The kit includes all the required reagents for fragmentation, library prep, and magnetic bead-based cleanup, and it is compatible with your NEXTFLEX® barcodes of choice. This DNA-seq library prep kit is highly flexible in terms of sample requirements, accommodating a range of sample types and sample input amounts from 1 ng to 1 µg to quickly and efficiently generate high-quality libraries for Illumina®sequencing.
Unprecedented Data Security in Sequencing Applications
The NEXTFLEX® Unique Dual Index Barcodes are barcoded adapters, for sequencing on Illumina® platforms, that provide unprecedented data security in sequencing applications. Multiplexing with NEXTFLEX® Unique Dual Index Barcodes drastically increases scale while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane. These barcoded adapters can be used with single read or paired-end read sequencing, and are compatible with NEXTFLEX® library prep kits designed for Illumina® sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
Start your Preimplantation Genetic Testing with the Right DNA Amplification
Genomic analysis platforms require at least 1 ng of DNA and single cells contain only 6 pg of genomic DNA. Whole genome amplification is used to replicate the complete genome to generate sufficient DNA for analysis. The accurate interpretation of single cell data relies on the ability of the whole genome amplification to comprehensively and reliably amplify the genome. The DOPlify® kit has been optimized specifically for single cell whole genome amplification. The kit is suited not just to single cells but to any low template sample, including cell free DNA and small numbers of cells.
For research use only. Not for use in diagnostic procedures.