RT-PCR and NGS Solutions for SARS-CoV-2 Mutation Identification

Recent reports of potentially more dangerous SARS-CoV-2 variants such as the B.1.1.7 lineage [UK] and B1.351 lineage [S. Africa] highlights the need for surveillance to quickly identify changes in the viral genome and contain the spread. These variants are of particular concern because they may spread faster and/or may cause more severe disease. Additionally, variants may impact the performance of current diagnostic approaches or the effectiveness of vaccines.

RT-PCR is the gold standard tool for the diagnosis COVID-19, due to the inherent benefits of accuracy, sensitivity, speed, and scalability. However, these first-tier diagnostic assays provide limited information about SARS-CoV-2 variants in the sample. Second tier assays can provide information on variants by using a portion of the sample determined to be positive using the first-tier assay.

PerkinElmer offers two SARS-CoV-2 second-tier assay options, giving labs the flexibility to implement one or both solutions based on the information being sought and workflow considerations.

SARS-CoV-2 second-tier assay options for variant identification

RT-PCR SARS-CoV-2 Mutation Panel

The PKamp VariantDetect SARS-CoV-2 RT-PCR assays will enable labs to identify mutations specifically associated with B.1.1.7, B.1.351 and B.1.1.248, which are the predominant variants known today. Our team is closely tracking literature and trends in partner labs across the globe to identify and design assays for detecting emerging mutations related to increased penetration, vaccine efficacy, immune response, and therapy. Assays for these emerging mutation targets of interest will be available upon request.

Whole Genome Sequencing of SARS-CoV-2

The NEXTFLEX® Variant-Seq™ SARS-CoV-2 WGS workflow is a genome-wide, unbiased technique which can identify all SARS-Cov-2 genetic changes without requiring prior knowledge of variants. The high resolution of NGS identifies all current and future variants of concern and is used to understand the epidemiology of SARS-CoV-2 infection.

PKamp VariantDetect Kit NEXTFLEX® Variant-Seq Kit
Platform RT-PCR NGS
Result Information About KNOWN Predominant Variants Information About ALL Variants
Sample-to-Answer Time   Faster Slower
Reagent Cost Lower Higher
Data Analysis Complexity Lower Higher

Let Us Help You Choose the Variant Identification Solution Best Suited for Your Needs

For research use only. Not for use in diagnostic procedures.