FUTURE-PROOFING COVID-19 DETECTION
The Omicron variant does not impact the performance of the PerkinElmer SARS-CoV-2 Plus RT-qPCR Reagent kit based on in silico assessment against sequences published in the GISAID database.
PerkinElmer® SARS-CoV-2 Plus (N/ORF1ab/E/S 69_70del) RT-qPCR Reagent Kit
The PerkinElmer® SARS-CoV-2 Plus RT-qPCR Reagent kit (Cat # 3515-0010) is CE-IVD marked and intended for in vitro diagnostic use. This assay provides reliable and high-quality results for the clinical detection of COVID-19 in human oropharyngeal and nasopharyngeal swabs.
Sensitive & Specific Nucleic Acid-based Detection
of SARS-CoV-2 ORF1ab and N Genes
The SARS-CoV-2 Plus RT-qPCR Reagent kit has a limit of detection 20 copies/rxn for the SARS-CoV-2 ORF1ab, N, and E genes. It does not show cross reactivity with pathogens recommended by the WHO (High priority organisms) including:
- Human coronavirus (229E, OC43, HKU1, and NL63)
- SARS-coronavirus (plasmid)
- MERS-coronavirus (plasmid)
- Adenovirus (e.g. C1 Ad. 71)
- Enterovirus (e.g. EV68)
- Rhinovirus
- Influenza A virus
- Influenza B virus
- Respiratory syncytial virus
- Parainfluenza virus (1, 2, 3, 4a)
- Human Metapneumovirus (hMPV)
- Rhinovirus
- Mycobacterium tuberculosis
- Chlamydia pneumoniae
- Haemophilus influenzae
- Legionella pneumophila
- Bordetella pertussis
- Mycoplasma pneumoniae
- Pneumocystis jirovecii
- Candida albicans
- Streptococcus pyogenes
- Streptococcus saliva
- Pseudomonas aeruginosa
The PerkinElmer® SARS-CoV-2 RT-qPCR Reagent kit includes both positive and negative controls to prevent the reporting of incorrect results.

Nucleic Acid Extraction Expertise
Nucleic acid quality is of major importance when conducting molecular analysis since factors causing degradation and the presence of impurities and enzymatic inhibitors can have a significant impact on the quality of data. The chemagic™ technology addresses the challenges associated with extraction while offering high recovery of clean nucleic acids suitable for demanding downstream applications.
The chemagic™ Viral DNA/ RNA 300 Kit H96 (cat # CMG-1033-S) and the chemagic™ 360 instrument is recommended for the isolation of nucleic acids for use with the SARS-CoV-2 Plus RT-qPCR Reagent kit.
KIT CONTENTS
- Cov2 Reagent C
- Cov2 Enzyme mix NR4
- Cov2 Positive control 4
- Cov2 Negative control 4
SARS-CoV-2 Plus (N/ORF1ab/E/S 69_70del) RT-qPCR Reagent kit (Cat # 3515-0010) contains reagents for 96 reactions.
SARS-CoV-2 Plus (N/ORF1ab/E/S 69_70del) RT-qPCR Reagent kit (Cat # 3515-0010) contains reagents for 96 reactions. The shelf life of all reagents is -30 to -16°C protected from light until expiry date stated on the vial labels.