SIMPLIFYING THE PROCESSING OF SALIVA SAMPLES & INCREASING TESTING SENSITIVITY

Optimized Saliva Workflow for SARS-CoV-2 Testing

Saliva is desirable as a specimen type for SARS-CoV-2 testing because sample collection is much easier and less invasive than other sample types. PerkinElmer has optimized the entire workflow for SARS-CoV-2 testing of saliva samples to simplify sample processing and increase the sensitivity of the test.

Optimized Saliva Workflow for SARS-CoV-2 Testing
RNA isolation for SARS-CoV-2 NGS

Saliva Collection

1-5 mL of saliva are collected in a sterile, leak-proof screw cap container.

Sample Homogenization

Homogenization is an important step in PerkinElmer’s optimized saliva detection workflow. Adding a homogenization step with the BeadBoost Bead Mill homogenizer prior to viral RNA isolation simplifies the processing of saliva samples and increases the sensitivity of SARS-CoV-2 detection in these samples.

RNA isolation for SARS-CoV-2 NGS
RNA-seq library prep for SARS-CoV-2

Sample Reformatting & Pooling

The JANUS® G3 primary sample reformatter provides safe and traceable barcode scanning and reformatting of up to 192 saliva samples per run into various destination labware to support sample homogenization and viral RNA extraction.

A plug-and-play method automating pooling prior to viral RNA isolation on the JANUS® G3 primary sample reformatter simplifies pooling protocols and reduces human error.

Isolation of Viral RNA

Nucleic acid quality is a critical factor for SARS-CoV-2 testing. Isolating viral RNA form saliva can be challenging due to the abundance of impurities in saliva. The presence of these impurities, enzymatic inhibitors, and degraded nucleic acids all can have a negative impact on real-time PCR testing. PerkinElmer’s chemagen nucleic acid extraction technology overcomes these challenges to enable robust and reproducible extraction of high-yield and high-quality RNA from human saliva.

The PerkinElmer chemagic Viral DNA/ RNA 300 kit H96 and the chemagic 360 instrument are recommended for fast, high quality RNA extraction for use in PCR based detection of SARS-CoV-2.

Increase your SARS-CoV-2 library prep throughput
PCR assay setup for saliva workflow

PCR Assay Setup

The JANUS® G3 PCR workstation enables traceable and reproducible full walk-away RT-PCR sample prep while reducing the risk of hands-on errors in high-throughput SARS-CoV-2 testing. The JANUS® G3 PCR workstation is shipped with standardized sample prep protocols and can be configured to automate any SARS-CoV-2 PCR assay.

Citations:

Sahajpal, N. S., Mondal, A. K., Ananth, S., Njau, A., Ahluwalia, P., Chaubey, A., . . . Kolhe, R. (2020). SalivaAll: Clinical validation of a sensitive test for saliva collected in healthcare and community settings with pooling utility for SARS-CoV-2 mass surveillance. doi:10.1­­­101/2020.08.26.20182816

Contact Us to Optimize Your Saliva SARS-CoV-2 Workflow

For research use only. Not for use in diagnostic procedures.