QUICKLY IDENTIFY KNOWN SARS-COV-2 MUTATIONS WITH RT-PCR

PKamp VariantDetect SARS-CoV-2 RT-PCR Assay

The PKamp VariantDetect SARS-CoV-2 RT-PCR assays utilize real-time RT-PCR multiplexing technology for simultaneous qualitative detection and differentiation of several known SARS-CoV-2 mutations. The assays provide information about SARS-CoV-2 mutations allowing labs to identify known variants from positive samples.

  • Identify mutations in a positive sample within hours
  • Leverage existing RT-PCR workflow used for 1st tier testing
  • Cost-effective 2nd tier mutation typing using extracted sample available from 1st tier testing
Rapid detection of SARS-CoV-2 mutations
For research use only. Not for use in diagnostic procedures.

Detectable SARS-CoV-2 Mutations

The PKamp VariantDetect SARS-CoV-2 RT-PCR assay allows labs to promptly perform secondary testing on positive COVD-19 samples to identify mutations in the SARS-CoV-2 virus in the samples.

Alpha VOC (B.1.1.7) : Amongst other mutations, a 69/70 deletion in the spike protein gene has been reported in the Alpha variant.* This variant is of concern because it is associated with increased transmissibility.

Beta VOC (B.1.351): Multiple mutations have been reported in the spike protein for the Beta variant, including E484K and N501Y.* B.1.351 does not have the residue 69-70 deletion observed in B.1.1.7, but a deletion at residues 242-244 has been reported.**

Gamma VOC (P.1): Three mutations in the spike protein receptor binding domain: K417T, E484K, and N501Y have been reported in the Gamma variant. This variant also does not contain the deletion at 69/70 in the spike protein gene.* The P.1 variant may increase transmissibility or propensity for SARS-CoV-2 to re-infect individuals.

Delta VOC (B.1.617):  There are eight mutations in the Delta virus’s spike protein including the common signature mutations D111D, G142D, L452R, E484Q, D614G and P681R in the spike protein coding region, including within the receptor binding domain (RBD).

PKamp™ VariantDetect™ SARS-CoV-2 RT-PCR Assay Workflow

Assay Combinations

Target Mutations Designed to Differentiate Mutations Associated With Part Number(s)
Spike L452R

Spike E484Q

Spike E484K

Lineage B.1.617 SDX-56617

Assay Combinations

Target Mutations Designed to Differentiate Mutations Associated With Part Number(s)
Spike H69-V70 deletion

Spike L242-L244 deletion

ORF1ab S3675-F3677 deletion

B.1.1.7, B.1.351, and P.1 variants 4224-0010

Mutation/Variant Matrix

Reported Associations Wuhan B.1.1.7 B.1.351 P.1
N-gene control + + + +
Spike H69-V70 deletion +
Spike L242-L244 deletion + + +
ORF1ab 3675 – 3677 deletion + + +

Assay Combinations

Target Mutations Designed to Differentiate Mutations Associated With Part Number(s)
Spike H69-V70 deletion

Spike N501Y

B.1.1.7 and B.1.3.5.1/P1 SDX-56611
SDX-56612
SDX-56613

Mutation/Variant Matrix

Reported Associations Wuhan B.1.1.7 B.1.351/P.1
N-gene control + + +
Spike H69-V70 deletion +
N501Y + +

Assay Combinations

Target Mutations Designed to Differentiate Mutations Associated With Part Number(s)
Spike H69-V70 deletion

Spike E484K

B.1.1.7 and B.1.3.5.1/P1 SDX-56611
SDX-56613
SDX-56614

Mutation/Variant Matrix

Reported Associations Wuhan B.1.1.7 B.1.351/P.1
N-gene control + + +
Spike H69-V70 deletion +
E484K +

Assay Combinations

Target Mutations Designed to Differentiate Mutations Associated With Part Number(s)
Spike H69-V70 deletion

Spike N501Y

Spike E484K

B.1.1.7 and B.1.3.5.1/P1 SDX-56611,
SDX-56613,
SDX-56616

Mutation/Variant Matrix

Reported Associations Wuhan B.1.1.7 B.1.351/P.1
N-gene control + + +
Spike H69-V70 deletion +
N501Y + +
E484K +

Additional mutations that may of interest: N501Y, E484K, P681H, D614G, K417N, A222V, Y453F, N439K, K417T, A570D, T716I, S982A, A1708D, A701V, Q27stop, delY144, D80A, L18F, R246I, and D215G. Please contact us about assays for targeting these mutations. Our team is closely tracking literature and trends in partner labs across the globe to identify and design assays for detecting emerging mutations related to increased penetration, vaccine efficacy, immune response, and therapy.

PerkinElmer’s Complete SARS-CoV-2 RT-PCR Workflow

PerkinElmer enables labs to simplify secondary SARS-CoV-2 variant detection while maintaining sample integrity and avoid cross contamination of SARS-CoV-2 PCR samples by providing modular and scalable automated workflow solutions. PerkinElmer’s RT-PCR-based SARS-CoV-2 variant identification workflow provides automation with flexible throughput, and full traceability.

The assays are designed for real-time RT-PCR instruments with FAM, ROX, Cy®5 and/or HEX/VIC channels.

* CDC Emerging SARS-CoV-2 Variants. March 2, 2021.

** ECDC Risk related to the spread of new SARS-CoV-2 variants of concern in the EU/EEA – first update, January 21, 2010.

Contact us to Quickly identify known SARS-CoV-2 mutations
For research use only. Not for use in diagnostic procedures.