Whole Genome Sequencing of the SARS-CoV-2 Genome
As of March 2021, SARS-CoV-2 has infected 115 million people and caused over 2.56 million deaths.1 The discovery of particularly aggressive variants such as B.1.1.7 and B.1.351 has shown that monitoring for mutations associated with changes to infection outcome and transmission of SARS-CoV-2 is critical to ensure the success of vaccination programs and establish robust public health responses2. Once a sample is detected as positive for COVID-19 using an RT-PCR assay, the next question that needs to be answered is, does the sample contain a SARS-CoV-2 Variant of Concern (VOC). NGS is ideal for this as its high resolution enables it to identify all mutations, known and unknown, providing insights to SARS-CoV-2 infection and transmission.
The NEXTFLEX® Variant-Seq™ SARS-CoV-2 workflow is optimized to quickly, easily, and affordably identify all mutations in a SARS-CoV-2 PCR-positive sample. It utilizes an amplicon-based target enrichment workflow which offers many advantages over hybridization capture-based protocols, most notably speed, scalability, and cost.
Track & Identify SARS-CoV-2 Variants
The NEXTFLEX® Variant-Seq™ SARS-CoV-2 Kit has optimized the identification and tracking of SARS-CoV-2 variants. Sample preparation has been simplified, throughput has been increased, and multiple automation solutions offer flexibility based on your lab’s needs. Along with a complete, sample-to-answer workflow, PerkinElmer is here to help labs meet their SARS-CoV-2 variant detection challenges.
The NEXTFLEX® Variant-Seq™ SARS-CoV-2 Kit is powered by COSMOSID® software, converting sequencing data into a concise sample report within minutes. These reports are compliant with the CDC requirements for tracking variants. The Pangolin lineage, used by the CDC to track variants, is included in the reports. Additionally, the SARS-CoV-2 consensus created using the report can be easily submitted to NCBI and GISAID.