NGS WORKFLOWS FOR THE IDENTIFICATION OF SARS-COV-2 VARIANTS

One of the primary advantages next-generation sequencing (NGS) provides over other methods of analysis is that it is an effective and high-throughput solution for screening multiple samples and detecting viruses without prior knowledge of an infectious agent. Among its core capabilities, NGS workflows can be used to detect SARS-CoV-2 virus, perform surveillance and epidemiological studies, track mutational changes in the virus, enable unbiased pathogen discovery, and analyze potential susceptibility and human response to SARS-CoV-2 infection.

Identifying SARS-CoV-2 Variants

Multiple SARS-CoV-2 variants are circulating globally, some of which are reported to spread more easily and faster than others. Now you can sequence 6,000 SARS-CoV-2 libraries in a single flow cell using the high-throughput NEXTFLEX® Variant-Seq™ SARS-CoV-2 workflow optimized to quickly, easily, and affordably identify all mutations, including the Delta Variant of Conceren (VOC), in a PCR-positive sample.

Variant-Seq Kit

For research use only. Not for use in diagnostic procedures.

AUTOMATED WORKFLOWS SIMPLIFY NGS ANALYSIS OF SARS-COV-2

Automation supports a lab’s ability to rapidly and safely perform NGS analysis of SARS-CoV-2 samples. PerkinElmer’s workflow automates nucleic acid isolation right through to library-prep analysis, simplifying library preparation, mitigating risk of lab-acquired infections, increasing throughput, and reducing variability.

Streamlined WGA Workflow Simplifies Variant Identification While Increasing Throughput

It’s important to know which variant is in a positive COVID-19 sample. The NEXTFLEX® Variant-Seq™ SARS-CoV-2 kit simplifies variant identification. After identifying a positive COVID-19 sample, it simplifies the identification of SARS-CoV-2 mutations with an amplicon-based WGA workflow using ARTIC v3 primer pools. The protocol is streamlined by eliminating a new extraction and quantitation prior to reverse transcription. Throughput is maximized and sequencing costs are minimizde with 1,536 unique dual index barcodes up to 6,000 sample can be run on a single  Illumina® NovaSeq® flowcell.

RNA-seq library prep for SARS-CoV-2
Increase your SARS-CoV-2 library prep throughput

Automate Your Variant Detection

Pre-programmed protocols are available automating the NEXTFLEX® Variant-Seq SARS-CoV-2 kit on the Sciclone® G3 NGSx, Sciclone® G3 NGSx iQ and JANUS® G3 liquid handlers. These multiple automation options offer labs the flexibility to meet their specific throughput and hands-on time needs.

Library QC

The LabChip® GX Touch nucleic acid analyzer using the NGS 3K assay delivers automated analysis of the sizes and profiles of NGS libraries for required quality assessment prior to sequencing. With flexible throughput options and multiple data visualization choices, the LabChip® GX Touch nucleic acid analyzer simplifies library QC.

LabChip Instrument
Contact Us to Improve Your SARS-CoV-2 NGS Workflows

For research use only. Not for use in diagnostic procedures.