Analysis of SARS-CoV-2
PerkinElmer’s NEXTFLEX® Rapid Directional RNA-seq kit 2.0 is ideal for coronavirus-related NGS studies including library preparation for targeted sequencing, whole genome and shotgun sequencing, and expression analysis. It provides high coverage uniformity, low duplication rates, strand specificity with a streamlined, automated protocol enabling rapid analysis of SARS-CoV-2 samples.
Additionally, the 1, 536 NEXTFLEX® UDI barcodes, which can be used with the NEXTFLEX® Rapid Directional RNA-seq kit 2.0 or other library preparation protocol, enable the multiplexing up to 1,536 samples onto one Illumina® flow cell significantly reducing the sequencing cost and time.
The ability to multiplex a large number of samples in a single run is one of the biggest benefits of using an amplicon-based approach for epidemiological sequencing studies of the SARS-CoV-2 virus as it significantly decreases the turnaround time and cost of analysis. With the ability to multiplex up to 1,536 amplicon libraries in a single run, the NEXTFLEX® DNA-seq kits along with the NEXTFLEX® UDI barcodes deliver a simple, cost-effective solution for adding adapters compatible with Illumina® sequencing to SARS-CoV-2 amplicon pools. For amplicon pools which need to be fragmented, we recommend using the NEXTFLEX® Rapid XP DNA-seq kit. For those that do not need fragmentation, we recommend using the NEXTFLEX® Rapid DNA-seq kit 2.0.