ENABLING ACCURATE NON-INVASIVE PGT-A

PG-Seq Rapid Non-Invasive Kit with High Correlation to Biopsied Embryos

for Illumina® & Thermo Fisher® Ion Torrent® Instruments

The PG-Seq™ Rapid Non-invasive PGT kit has been specifically developed to analyze picogram quantities of DNA (low template DNA) from spent embryo culture media or blastocoelic fluid samples for non-invasive preimplantation genetic testing. Optimization of the kit was performed using non-invasive samples provided to PerkinElmer from 15 labs around the world, each using different embryology protocols and accuracy was assessed by the degree of correlation to embryo biopsies tested with a range of commercially available PGT-A kits.

The kit is a modified version of the PG-Seq™ Rapid kit, which uses a two-step PCR to whole genome amplify and then attach indexes and sequencer-specific adapters to the template DNA, in a fast, 3-hour sample preparation workflow. The kit is available in an Illumina® or Thermo Fisher® Ion Torrent® sequencer compatible formats. From DNA to data analysis, the kit includes all reagents required for whole genome amplification, indexing, plus PG-Find™ analysis software for automatic calling of aneuploidy and copy number variants.

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PG-SEQ RAPID NON-INVASIVE TECHNOLOGY BENEFITS

Example results from spent embryo culture media; 45,XY,-9 and 47,XX,+21, both concordant with embryo biopsy results.

“From what we’ve observed so far, the results look excellent, and we are looking forward to further developing our non-invasive PGT program.”

Manuel Viotti, PhD, Senior Scientist, Zouves Foundation for Reproductive Medicine, Zouves Fertility Center
Kit Contents

WGA REAGENTS

  • PCR-grade H20
  • WGA Polymerase
  • WGA PCR Buffer
  • Round 1 Primer
  • dNTPs

INDEXED PRIMERS

  • Indexed Primers

ADDITIONAL REAGENTS

  • Purification Beads
  • Resuspension Buffer

Required materials not provided
Pre-PCR Laboratory requirements

  • Laminar flow cabinet
  • Minicentrifuge
  • Pipettes (2, 10, 20, 100, 200, 1000 µl)
  • Cold block (4°C)
  • Thermocycler (with hotlid & programmable ramp rate to 0.2°C/sec)
  • Pipette tips (low binding, barrier filter)
  • PCR thin walled reaction tube with flat cap (0.5mL or 0.2mL)
  • PCR-grade 96-well plate to suit thermal cycler
  • Molecular grade tubes (1.5mL)
  • 96-well plate centrifuge
  • Adhesive 96-well plate seals
  • Vortex

Post-PCR Laboratory requirements

  • Magnetic stand for 96-well plates
  • 96-well plate, to suit Magnetic stand
  • Adhesive 96-well plate seals
  • Absolute ethyl alcohol (EtOH, undenatured) to make 80% ethanol
  • Molecular grade water
  • Ice
  • Tris-HCl 200 mM (pH 7.0)
  • PhiX Sequencing Control v3 10 nM (cat # FC-110-3001) diluted to 20 pM (Illumina)
  • LabChip® GXII Touch nucleic acid analyzer and associated reagent kit (PerkinElmer)
  • Thermo Fisher® Qubit® Fluorometer and associated reagent kit (Thermo Fisher Scientific)
  • Sodium hydroxide 1N
  • Illumina® Sequencer (MiSeq® Instrument or MiniSeq® Instrument)
  • Illumina® Sequencer related consumables
  • Illumina® Sequencer reagent kit (select from):
    • Illumina® MiSeq® Reagent Kit v3 (150 cycle, cat # MS-102-3001)
    • Illumina® MiSeq® Reagent Micro Kit v2 (300 cycle, cat # MS-103-1002)
    • Illumina® MiniSeq® High Output Reagent Kit (75 cycles, cat # FC-420-1001)

Optional materials not provided

  • Agarose gel-electrophoresis apparatus
  • Electrophoresis power supply
  • UV transilluminator or gel documentation instrument
  • Multi-channel pipette
  • Multi-channel pipette reagent reservoirs

Catalog Numbers

PG-Seq™ Rapid Non-Invasive Kit for Illumina® NGS – 4328-0010
PG-Seq™ Rapid Non-Invasive Kit for Thermo Fisher® Ion Torrent™ NGS – 4329-0010

The PG-Seq Rapid Non-Invasive PGT Kit comes with sample preparation reagents in a 96-reaction kit and the PG-Find analysis software.

The shelf life of all reagents is 6 months when stored properly at -20°C. The PG-Seq Rapid Non-invasive PGT kit ships on ice packs.

Catalog Numbers
PG-Seq Rapid Non-Invasive Kit for Illumina® NGS – 4328-0010
PG-Seq Rapid Non-Invasive Kit for Thermo Fisher® Ion Torrent™ NGS – 4329-0010

For research use only. Not for use in diagnostic procedures.