- PCR-grade Water
- Cell Lysis Enzyme
- Cell Lysis Buffer
- WGA Polymerase
- WGA PCR Buffer
REQUIRED MATERIALS NOT PROVIDED
- 0.2 or 0.5 mL PCR tubes
- Single blastomeres, polar bodies, trophoblastic cells, amniocytes, lymphocytes, or cultured cells
- PCR thermocycler
How many reagents are included in the DOPlify® kit?
Cell lysis buffer, Cell lysis enzyme, PCR-grade H20, Primer, Polymerase and PCR buffer.
How many reactions do I get in a single DOPlify® kit?
How long is the protocol?
~3 hours total (including hands on time and incubations).
What thermocycler do you recommend for PCR?
Any thermocycler that has a programmable ramp rate will be fine.
At what temperature do I store the kit components?
DOPlify® kit should be stored at -20°C in a clean/pre-PCR lab.
What buffer should I use amplify my samples in?
Recommended cell transfer buffers include 10 mM Tris-HCl (pH 8.0) (no EDTA) and PBS (Mg2+, Ca2+ free and BSA free) in 0.2 ml or 0.5 ml PCR tubes.
Publications that Cite Using the DOPlify® Kit for Whole Genome Amplification
Deleye, L., Tilleman, L., Plaetsen, A. V., Cornelis, S., Deforce, D., & Nieuwerburgh, F. V. (2017). Performance of four modern whole genome amplification methods for copy number variant detection in single cells. Scientific Reports, 7(1). doi:10.1038/s41598-017-03711-y.
Deleye, L., Plaetsen, A. V., Weymaere, J., Deforce, D., & Nieuwerburgh, F. V. (2018). Short Tandem Repeat analysis after Whole Genome Amplification of single B-lymphoblastoid cells. Scientific Reports,8(1). doi:10.1038/s41598-018-19509-5.
Mai, A. D., Harton, G. L., Quang, V. N., Van, H. N., Thi, N. H., Thuy, N. P., . . . Quoc, Q. T. (2020). Development and clinical application of a preimplantation genetic testing for monogenic disease (PGT-M) for beta thalassemia in Vietnam. Journal of Assisted Reproduction and Genetics. doi:10.1007/s10815-020-02006-y.
Plaetsen, A. V., Deleye, L., Cornelis, S., Tilleman, L., Nieuwerburgh, F. V., & Deforce, D. (2017). STR profiling and Copy Number Variation analysis on single, preserved cells using current Whole Genome Amplification methods. Scientific Reports, 7(1). doi:10.1038/s41598-017-17525-5.
The DOPlify® Kit contains reagents for amplifying 50 samples. Ships on on ice packs and remain stable even if the ice packs have thawed and the reagents reach room temperature. Upon receipt, store the kit at -20°C in a constant temperature freezer (not frost free).
DOPlify® Kit – 50 rxn – 4321-0010
DOPlify® Kit – 15 rxn – 4322-0010