TARGETED DETECTION OF THE MONKEYPOX VIRUS
PKamp™ Monkeypox Virus Real-time PCR RUO Kit V1
The PKamp™ Monkeypox Real-time PCR RUO Kit V1 is a real-time PCR multiplexed test intended for the qualitative detection of Monkeypox virus (MPXV) DNA from extracted nucleic acid. The kit does not detect Variola virus (VARV) DNA and other non-variola Orthopoxvirus (NVAR) DNA. The genus Orthopoxvirus contains four species that infect humans: variola (VARV), monkeypox (MPXV), vaccinia (VACV) including buffalopox, and cowpox (CPXV).
The PKamp™ Monkeypox Real-time PCR RUO Kit V1 includes both positive and negative internal controls for accurate reporting of results. An additional primer/probe set included to detect the endogenous control gene RNase P enables monitoring of sample collection from human biological material and extraction efficiency.
Specific: Targets Monkeypox virus’ F3L gene
Sensitive: Detects at least 20 copies per PCR reaction
Seamless: Compatible with our sample prep workflow
“This Monkeypox assay is a surgical assay, as I said, a piece of art, works so clean.”
Dr. Alberto Paniz-Mondolfi, Icahn School of Medicine at Mount Sinai
Nucleic Acid Extraction Expertise
We excel in delivering high-quality molecular detection workflows by combining expertise in RT-PCR assay and nucleic acid extraction as most recently demonstrated during the COVID-19 pandemic. Nucleic acid quality is critical when conducting molecular analysis since degradation, impurities and enzymatic inhibitors can have a significant impact on the quality of data.The chemagic™ technology addresses the challenges associated with extraction by offering high recovery of clean nucleic acids suitable for demanding downstream applications. The chemagic™ Viral DNA/ RNA 300 Kit H96 (cat # CMG-1033-S) and the chemagic™ 360 instrument is recommended for the isolation of nucleic acids for use with the PKamp™ Monkeypox Real-time PCR RUO Kit V1.
Flexible Automation
The JANUS® G3 primary sample reformatter provides safe and traceable barcode scanning and reformatting of samples to support viral nucleic acid extraction. The JANUS® G3 PCR Workstation enables traceable and reproducible real time PCR sample preparation for high-throughput detection using standardized protocols. The PKamp™ Monkeypox Real-time PCR RUO Kit V1 works with the commonly available real-time PCR instruments on the market including the EONIS™ Q instrument.
PERKINELMER’S qPCR WORKFLOW
PKamp™ Monkeypox Virus Real-time PCR RUO Kit V1
SDX-62669 (15 µL PCR)
- Reagent A
- Enzyme Mix
- Negative Control
- MPXV Reagent B1
PKamp™ Monkeypox Virus V1 Positive Controls
SDX-62670 (20 Tests for 15 µL PCR)
- MPXV Positive Control 1
Michel, J., Targosz, A., Rinner, T., Bourquain, D., Brinkmann, A., Sacks, J. A., Schaade, L., & Nitsche, A. (2022). Evaluation of eleven commercially available PCR kits for the detection of monkeypox virus DNA. https://doi.org/10.1101/2022.10.14.22281096
Each kit contains 192 tests. The shelf life of all reagents is 24 months when stored properly. Store the assay at -25℃ to -15℃ for long-term storage.