PerkinElmer® SARS-CoV-2 Nucleic Acid Detection Kit (RUO)

Streamline your laboratory developed SARS-CoV-2 testing with the PerkinElmer® SARS-CoV-2 Nucleic Acid Detection Kit (RUO), a PCR assay using fluorescent-labeled probes specific to the 2019 coronavirus (SARS-CoV-2) open reading frame 1ab (ORF1ab) and nucleocapsid protein (N) genes.

As described in the kit insert, PerkinElmer’s RT-PCR assays target two specific genomic regions of SARS-CoV-2: the nucleocapsid (N) gene and ORF1ab. Thus mutations in spike protein, as seen in the B.1.1.7 and B.1.351 lineages, do not impact the safety and effectiveness of PerkinElmer’s assays.

  • Specific: Detection of SARS-CoV-2 ORF1ab and N genes

  • Sensitive: Limit of detection of 7.14 to 27.24 copies/mL

  • Flexible: Compatible with a variety of sample types

  • Reliable: Performance verified with case studies from outbreak’s point of origin

  • Automated: Options available to meet a broad range of sample throughput

For research use only. Not for use in diagnostic procedures.

Sensitive & Specific Nucleic Acid-based Detection
of 2019-nCoV ORF1ab and N Genes

The PerkinElmer® SARS-CoV-2 Nucleic Acid Detection kit (RUO) has a limit of detection 20 copies/mL for the SARS-CoV-2 ORF1ab and N genes. It does not show cross reactivity with common respiratory tract pathogens and blood stream pathogens including:

  • Human coronavirus (229E, OC43)
  • SARS coronavirus (plasmid)
  • MERS coronavirus (plasmid)
  • Adenovirus (3, 2, 31, 37, 51 types)
  • Enterovirus (A, D)
  • Rhinovirus (A, B)
  • Influenza A virus
  • Influenza B virus
  • Influenza A H1N1 virus (2009)
  • Seasonal influenza virus (H1N1)
  • Seasonal influenza virus (H3N2)
  • Respiratory syncytial virus
  • Parainfluenza virus
  • Measles virus
  • Mumps virus
  • Human cytomegalovirus
  • Chlamydia pneumoniae
  • Mycoplasma pneumoniae
  • Haemophilus influenzae
  • Staphylococcus aureus
  • Streptococcus pyogenes
  • Streptococcus saliva
  • Hepatitis A virus
  • Hepatitis B virus
  • Hepatitis C virus
  • EB virus
  • Herpes simplex virus type I
  • Herpes simplex virus type II
  • Human immunodeficiency virus type I (HIV-1)
  • Human immunodeficiency virus type II (HIV-2)

The PerkinElmer® SARS-CoV-2 Nucleic Acid Detection kit (RUO) includes positive, negative, and internal process controls to prevent the reporting of incorrect results.

Sample Types For SARS-CoV-2 Detection

This assay can be used to identify SARS-CoV-2 ORF1ab and N genes in 400 µL of the following sample types:

  • Oropharyngeal swab
  • Nasopharyngeal swab

Complete Workflow Solutions

Complete modular solutions are available to meet your automation needs. PerkinElmer enables labs to serve their customers’ needs by offering modular, tested workflow solutions providing high quality nucleic acid extraction, automation with flexible throughput, and sample process traceability ensuring the accurate identification of samples.

SARS-COV-2 Workflow Solution

Nucleic Acid Extraction Expertise

Nucleic acid quality is critical for molecular analyses as factors causing sample degradation and impurities such as enzymatic inhibitors can have a significant negative impact on the quality of results. PerkinElmer’s chemagen™ nucleic acid extraction technology addresses these challenges and offers high recovery of clean nucleic acids suitable for demanding downstream applications.

The PerkinElmer chemagic Viral DNA/ RNA 300 Kit H96 and the chemagic 360 instrument are recommended for fast and high quality RNA extraction for use in PCR-based detection of SARS-CoV-2.

PCR Assay Setup

To meet high-throughput demands and reduce human errors, the JANUS® G3 workstation has been optimized to rapidly and accurately setup your SARS-CoV-2 PCR assay. Standardized and tested automated SARS-CoV-2 PCR set-up methods deliver easy-to-use automation allowing you to:

  • Decrease pipetting errors
  • Decrease lab staff workload
  • Reduce hands-on-time
  • Deliver full traceability of reagents, samples, volumes, and pipetting steps


  • nCoV reagent A
  • nCoV reagent B
  • nCoV enzyme mixture
  • nCoV internal standard
  • nCoV positive control
  • nCoV negative control

Each kit contains 48 tests. The assay ships on dry ice.

Contact Us to Accelerate Your SARS-CoV-2 Testing

For research use only. Not for use in diagnostic procedures.