LASER PRECISION PROTEIN ANALYSIS

ProteinEXact™ Assay for Protein Quantitation and Sizing

Bring high sensitivity to your protein quantitation and sizing applications with the ProteinEXact™ assay for the LabChip® GXII Touch™ protein characterization system. The ProteinEXact™ assay is ideal for use in both upstream and downstream biotherapeutics and gene therapy workflows which require the utmost in precision. The high resolution and reproducibility of protein concentration analysis delivered by the ProteinEXact™ assay enables accurate, high throughput AAV purity characterization for recombinant viral production. Additionally it enables high-throughput quantification and characterization of asymmetrical bispecific antibodies.

With the analytical power and speed of the LabChip® GXII Touch™ protein characterization system and IntelliChip™ assay optimization technology, you can accelerate your protein development workflows with high-precision reproducibility, sensitivity, and an expanded sizing range.

  • Dependable reproducibility for quantitation (<10% CV) and sizing (<2% CV)
  • Broad sizing and quantitation range: 6.5-250 kDa and 10-2000 ng/μL
  • Highly sensitive: 0.2 ng/μL
  • Flexible: Run in batches of 48 or 96 samples at a time
  • Fast: Analysis in ~65 sec per sample

The LabChip® GXII Touch™ protein characterization system delivers comparable results to traditional capillary electrophoresis with 46X faster run times without compromising sensitivity, resolution or reproducibility.

ProteinEXact™ Assay Specifications
Sizing Range6.5 kDa - 250 kDa
Sizing Precision RSD (CV)<2%
Sizing Resolution≤10%
Linear Dynamic Range10-2000 ng/µL
Maximum Total Protein Concentration2 mg/mL
Sensitivity Limit of Detection (LOD)0.2 ng/µL
Quantitation Reproducibility<10%
Maximum Salt Concentration1M NaCl at pH 6.5 to 8.5
Chip Primes per Reagent Kit10
Chip Sample Lifetime400
Sample Analysis Time65 sec
Contact us to Add Precision to Your Protein Quantitation and Sizing
For research use only. Not for use in diagnostic procedures.

PROTEINEXACT™ ASSAY REPRODUCIBILITY & SENSITIVITY

ProteinEXact reproducibility

At both high and low concentration ranges, the ProteinEXact™ assay provides exceptional reproducibility at a CV less than 10%

ProteinEXact Sensitivity

ProteinEXact™ assay provides high sensitivity across an expanded sizing range (6.5 – 250 kDa) starting as low as 0.2 ng/µL

  • Smith, M. T., Zhang, S., Adams, T., Dipaolo, B., & Dally, J. (2017). Establishment and validation of a microfluidic capillary gel electrophoresis platform method for purity analysis of therapeutic monoclonal antibodies. Electrophoresis,38(9-10), 1353-1365. doi:10.1002/elps.201600519

The ProteinEXact™ assay reagent kit includes the following components:

REAGENTS

  • Dye Solution- 1 vial
  • Sample Buffer- 4 vials
  • ProteinEXact™ Gel Matrix- 3 vials
  • ProteinEXact™ Ladder- 2 vials
  • Marker- 2 vials
  • Wash Buffer- 4 vials

CONSUMABLES

  • Spin filters- 20
  • Cleaning cloth- 1
  • Swabs- 3