GLYCAN PROFILING & SCREENING, SUPER CHARGED

LabChip® Extended Range N-Glycan Assay

A high percentage of all biotherapeutics contain N-linked glycosylation. The impact of N-linked glycosylation on protein function can be significant. Thus, the characterization of N-glycan composition is critical for the biotherapeutic development process. PerkinElmer’s LabChip® Extended Range N-Linked Glycan assay enables the high-throughput sizing and quantification of N-linked glycans from protein biotherapeutics on the widely adopted LabChip® GXII Touch protein characterization system.

Glycoprotein Sizing, Glycoprotein Relative Quantification & Charge Variant N-Glycan Analysis

The LabChip® Extended Range N-Linked Glycan assay is ideal for rapid screening of N-linked glycan profiles by combining:

  • Plate based reagents in 96-well format for convenience and ease of use in automated liquid handlers such as the JANUS® G3 workstation from PerkinElmer
  • On-plate protein denaturation
  • Rapid enzymatic deglycosylation with PNGase F in 96 well format
  • Rapid electrokinetic separations and detection using LabChip® microfluidic chip technology
  • Per sample run time is under 45 seconds with a full 96 sample analysis in under 90 minutes
  • Automated peak detection and reporting of N-glycan relative abundance
  • Minimal sample consumption: Only 8 µL of sample required
  • Digital data format: Gel-like image, electropherogram, and tabular data formats with automated sample alignment, overlay, scaling and zooming features, allow users to conveniently share, store and archive data
  • The LabChip® Glycan Profiling assay supports both neutral glycan and charged N-linked glycan found in MAbs. Drop down menus allow you to easily choose between assays.
Contact us to Add Precision to Your Protein Quantitation and Sizing
For research use only. Not for use in diagnostic procedures.

LabChip® Glycan Profiling Assay Specifications

Concentration Range 1.25 – 7.5 ng/µL, (10 – 60 µg of mAb in total)
Concentration Sensitivity 1.0 ng of G0f, Man3, G1f, G2, and G2S2 standards
Deglycosylation >95% of N-linked glycans will be released from mAb
Sizing Reproducibility CV < 2.5%
Sample Volume Usable ≥ 8 µL
Run Time ≤ 45 seconds per sample
Chip Lifetime 400 samples
Samples per Chip Prep 192 samples/per prep (1,344 samples/reagent kit)
Chip Preps per Reagent Kit 7 HT preps (1,344 samples)

Pre-Plated Kits: The Next Advancement in High Throughput N-linked Glycan Sample Preparation

The LabChip® Extended Range N-Glycan assay includes pre-plated reagents to enable high-throughput sample preparation by greatly reducing hands-on time and total preparation time. In the standard manual procedure, users need to transfer samples and reagents between plates three times, every one or two hours. Using liquid handlers such as the JANUS® G3 or Zephyr® G3 NGS workstations, samples are transferred between three SBS compliant 96-well plates automatically. Glycan releasing and labeling are completed using pre-programed, automated incubation steps. Besides the 20 minutes savings of manual pipetting time, users will be able to walk away from the workstation for 4 hours and be more productive on other more important tasks without any interruptions.

Manual vs Automated Pre-Plated Extended Range N-linked Glycan Assay Workflows
sizing and quantification of N-linked glycans

Figure 1: The LabChip® Extended Range Glycan assay supports the sizing and quantification of N-linked glycans in concentrations from 1.25 ng/µl (left) to 7.5 ng/µl (right)

LabChip Glycan Profiling Workflow

GLYCAN RELEASE AND LABELING KIT, P/N CLS155434

  • Denaturing Plate
  • PNGase F Plate
  • Dye Plate

Glycan LabChip Reagent Kit, P/N 760525

  • Glycan Gel Matrix
  • Glycan Marker
  • Glycan Marker Diluent
  • Glycan Ladder (dry)
  • Glycan Ladder Diluent

CONSUMABLES

  • Cleaning Cloth (VWR, Cat. # 21912-046)
  • Swab ITW (Texwipe, Cat. # TX758B)
  • Ladder Tubes (Not sold separately)
  • Buffer Tubes (Not sold separately)

HIGH RESOLUTION LABCHIP® CHIPS

  • HT High Resolution LabChip® (for use with LabChip® GXII Touch HT Instrument) 760524
  • Loebrich, S., Clark, E., Ladd, K., Takahashi, S., Brousseau, A., Kitchener, S., Herbst, R., & Ryll, T. (2018). Comprehensive manipulation of glycosylation profiles across development scales. MAbs, 11(2), 335–349. https://doi.org/10.1080/19420862.2018.1527665.