ACCURATE SIZING, QUANTIFICATION & QUALITY
CONTROL OF DNA, RNA & NGS LIBRARIES
LabChip® GX Touch™ Nucleic Acid Analyzer
DNA and RNA quantitation and sizing can be done in seconds using automated capillary electrophoresis separation. The LabChip® GX Touch™ nucleic acid analyzer’s microfluidic technology generates reproducible, high-resolution data and is optimal for:
- Fast, high-throughput QC analysis of NGS libraries for detection of mutations associated with SARS-CoV-2
- NGS library preparation (smear and fragment analysis) and quality control
- RNA and DNA fragment analysis (including cell-free DNA, DNA isolated from FFPE samples, and PCR-free libraries)
- Quantitation and qualification for CRISPR fragment analysis
- Screening nucleic acid source material for SARS-CoV-2 vaccine development
- Characterization and QC of small RNA molecules and CRISPR/Cas9 gRNA
- Multiplex Detection of SARS-CoV-2 Multiplex Detection of SARS-CoV-2 Variants of Concern using ARMS-PCR
The Comprehensive Solution for Genomic Sizing & Quantitation
- Quantitate DNA and RNA sample integrity hours faster than agarose gels
- Maximize rare or low-concentration samples like cfDNA, PCR-free libraries, and ChIP-seq libraries
- Accurate quantitation down to 25 pg/µL for DNA smears and 0.5 pg/µL for fragments
- NGS library prep analysis and QC
- Flexible throughput options make it cost-efficient – 96-well or 384-well (HT) format
- Data visualization options (electropherogram, virtual gel, or tabular report)
The LabChip GX Touch nucleic acid analyzer also supports a variety of DNA and RNA analysis, which provides unparallel genomic data quality with minimal input requirements. When the concentrations are low, and samples are precious, The LabChip® DNA High Sens and RNA Pico assays are ideal choice for reliable measurement and sample preservation. With complete analysis of genomic material in about 30 seconds, the LabChip GX Touch nucleic acid analyzer eliminates the nucleic acid quantitation workflow bottleneck. High-resolution analysis provides exact sizing and quantitation of DNA fragments and smears down to 5 bp from samples as small as 2 pg/µL.
The system offers a broad variety of DNA analysis options, including the NGS 3K assay, which provides unparalleled genomic data quality with minimal input requirements. This high degree of sensitivity also makes it the ideal instrument for gDNA and PCR-free DNA quantitation, where concentrations are low, samples are precious and their preservation is essential.
Increasing Speed and Throughput of SARS-CoV-2 Variant Detection Workflows
The capacity and speed of analysis make the LabChip® NGS 3K assay run on the LabChip® GX Touch™ nucleic acid analyzer an ideal component of high-throughput NGS-based variant detection workflows. Figure 1 illustrates examples of fully constructed library of CoV+ sample with pooled amplicon input at 200 ng (blue line) and 1200 ng (red line). It only takes a minute to examine NEXTFLEX® Variant Seq™ SARS-CoV-2 libraries, to ensure proper library sizing quality and exclusion of contaminating fragments.
- Minimal sample consumption to save constructed library for next sequencing steps
- Automated process for high measurement reproducibility and operation reliability
- Multiple digitized data formats for convenient data analysis, visualization, and sharing

Figure 1: Validation of NEXTFLEX® Variant Seq™ SARS-CoV-2 Libraries.
Libraries of CoV+ samples were analyzed using the LabChip® NGS 3K assay on the LabChip® GX Touch™ nucleic acid analyzer: 200 ng pooled amplicon input (blue) and 1200 ng pooled amplicon input (red).
Purity and Integrity Analysis of mRNA-based Vaccines
By using LabChip® RNA Assay, researchers at Merck developed an assay to characterize purity and integrity of mRNA vaccines. This fast, easy and efficient workflow helped speed up vaccine development and meet increased manufacturing high volume demand.
Height: 69 cm | 27 in
Width: 51 cm | 20 in
Depth: 49 cm | 19.5 in
Windows 10-compatible software
Assay | Reagent Kit | 24 Chip | HT Chip |
---|---|---|---|
DNA 1K | CLS760673 | CLS138948 | 760517 |
DNA 5K | CLS760675 | CLS138949 | 760435 |
DNA 12K | 760569 | CLS138948 | 760517 |
DNA High Sens | CLS760672 | CLS138948 | 760517 |
Genomic DNA | CLS760685 | CLS138948 | 760517 |
DNA NGS 3K | CLS960013 | CLS145331 | CLS144006 |
cfDNA | CLS157242 | CLS145331 | CLS144006 |
RNA | CLS960010 | CLS138949 | 760435 |
RNA Pico | CLS960012 | CLS138949 | 760435 |
Small RNA | CLS153530 | CLS138949 | 760435 |
The system offers a broad variety of DNA analysis options, including the NGS 3K assay and the cfDNA assay, which provide unparalleled genomic data quality with minimal input requirements. This high degree of sensitivity also makes it the ideal instrument for gDNA, cfDNA, and PCR-free DNA quantitation, where concentrations are low, samples are precious, and their preservation is essential.
Bagheri, H., Friedman, H., Shao, H., Chong, Y., Lo, C., Emran, F., . . . Peterson, A. (2018). TIE: A Method to Electroporate Long DNA Templates into Preimplantation Embryos for CRISPR-Cas9 Gene Editing. The CRISPR Journal,1(3), 223-229. doi:10.1089/crispr.2017.0020.
Chesnais, V., Ott, A., Chaplais, E., Gabillard, S., Pallares, D., Vauloup-Fellous, C., . . . Ginoux, E. (2018). Using massively parallel shotgun sequencing of maternal plasmatic cell-free DNA for cytomegalovirus DNA detection during pregnancy: A proof of concept study. Scientific Reports, 8(1). doi:10.1038/s41598-018-22414-6.
Costa, J-M., et al. (2018). Cell-free fetal DNA versus maternal serum screening for trisomy 21 in pregnant women with and without assisted reproduction technology: a prospective interventional study. Genetics in Medicine. doi:10.1038/gim.2018.4.
Faiz, A., Heijink, I. H., Vermeulen, C. J., Guryev, V., Berge, M. V., Nawijn, M. C., & Pouwels, S. D. (2018). Cigarette smoke exposure decreases CFLAR expression in the bronchial epithelium, augmenting susceptibility for lung epithelial cell death and DAMP release. Scientific Reports,8(1). doi:10.1038/s41598-018-30602-7.
Ford, L., Carter, G. P., Wang, Q., Seemann, T., Sintchenko, V., Glass, K., . . . Kirk, M. D. (2018). Incorporating Whole-Genome Sequencing into Public Health Surveillance: Lessons from Prospective Sequencing of Salmonella Typhimurium in Australia. Foodborne Pathogens and Disease, 15(3), 161-167. doi:10.1089/fpd.2017.2352.
Gergen, J., Coulon, F., Creneguy, A., Elain-Duret, N., Gutierrez, A., Pinkenburg, O., . . . Haspot, F. (2018). Multiplex CRISPR/Cas9 system impairs HCMV replication by excising an essential viral gene. Plos One, 13(2). doi:10.1371/journal.pone.0192602.
Giand et al. (2021) Early transmission of SARS-CoV-2 in South Africa: An epidemiological and phylogenetic report. International Journal of Infectious Diseases, 103, 234-241, doi.org/10.1016/j.ijid.2020.11.128.
Kim, K.W., et al. (2021) Respiratory viral co-infections among SARS-CoV-2 cases confirmed by virome capture sequencing. Sci Rep 11, 3934 doi.org/10.1038/s41598-021-83642-x.
Mogilevsky, M., Shimshon, O., Kumar, S., Mogilevsky, A., Keshet, E., Yavin, E., Heyd, F., and Karni, R. (2018) Modulation of MKNK2 alternative splicing by splice-switching oligonucleotides as a novel approach for glioblastoma treatment. Nucleic Acids Res. doi: 10.1093/nar/gky921.
Ong, J., Woldhuis, R. R., Boudewijn, I. M., Berg, A. V., Kluiver, J., Kok, K., . . . Brandsma, C. A. (2019). Age-related gene and miRNA expression changes in airways of healthy individuals. Scientific Reports,9(1). doi:10.1038/s41598-019-39873-0.
Raffaele, J., Loughney, J. W., & Rustandi, R. R. (2021). Development of a microchip capillary electrophoresis method for determination of the purity and integrity of mrna in lipid nanoparticle vaccines. ELECTROPHORESIS. https://doi.org/10.1002/elps.202100272.
Trolle, C., et al. (2016) Widespread DNA hypomethylation and differential gene expression in Turner syndrome. Scientific Reports (6). doi:10.1038/srep34220.
SOFTWARE
APPLICATION NOTES
- A dual-reference study design for understanding and improving AAV genome size analysis using ssDNA 7K ladder.
- Considerations in AAV Genome Integrity Analysis: Guidelines from the Use of ssDNA Ladder on LabChip® GX Touch™ Instrument
- An Environmentally Compliant Solution for Fluorescence-Labeled ssDNA Fragment Analysis via Microfluidic Capillary Electrophoresis
- LabChip cfDNA Assay High Throughput Analysis Cell-free DNA App Note
- Bioanalytical Evaluation of Single stranded DNA for Gene Therapy Applications A Reference Standard for Quality Control Workflow
- High Sensitivity & High Throughput Stability Assessment of mRNA in Solution & Lyophilized Solids using the LabChip® GXII Touch™ System
- Characterization of double-stranded DNA unwinding efficiency during isothermal amplification by LabChip® GX Touch™ Nucleic Acid Analyzer
- Quantitative Characterization of Enzymatic Nucleic Acid Modification by LabChip® GX Touch™ Nucleic Acid Analyzer: mRNA Capping, Phosphorylation & Adenylation
- CRISPR-Cas9 Genome-editing Efficiency Measured Accurately Using Fluorescently Labeled Single-Stranded DNA for Fragment Analysis with the LabChip® GX Touch™ Nucleic Acid Analyzer
- Multiplex Detection of SARS-CoV-2 Variants of Concern using ARMS-PCR on the LabChip® GX Touch™ Nucleic Acid Analyzer
- LabChip® GX Touch™ Small RNA Assay App Note
- Improving the Efficiency of Metagenomic Analysis of Stool Samples
- Automated NGS Library Preparation with Covaris® Focused-ultrasonicator Workflow Solution
- LabChip GX Touch Platform Applications in Epigenetic Studies
- Fast Genotyping of Genome-Edited Animals using Heteroduplex Mobility Assay and the LabChip GX Touch Instrument
- Determining Efficiency of On-Target CRISPR/Cas9 Genome Editing Using the NEB EnGen Mutation Detection Kit on LabChip GX Touch Technology
- Cost-effective Solution for Analyzing 24 samples or Less on the LabChip® GX Touch™ Nucleic Acid Analyzer
- Overcoming Challenges In Liquid Biopsy
- NGS 3K Assay for High Sensitivity Measurement of Cell-free DNA
FLYERS