Rapid Same-Day Workflow
Although hybridization capture has great advantages including minimized bias, stable and reliable data results from a variety of sample types, the complexity of the workflow and the long prep time have been obstacles to the users. The NEXTFLEX® Human CES Panel workflow significantly simplifies the process and reduces experiment time. Conventional CES takes 2-3 days to complete one sequencing experiment. With NEXTFLEX® Human CES Panel workflow, the entire experiment can be completed and the NGS run can be started on the same day.
Streamlined Workflow Reduces Costs & Experiment Time
Instruments such as vacuum concentrators, sonicators, and devices to characterize libraries are barriers against complete automation of core exome sequencing. The NEXTFLEX® Human CES panel does not require this instrumentation and alternatively uses enzymes and beads. The incorporation of normalization beads reduces turn-around time by up to three hours mostly due to eliminating the need for individual library quantification prior to the pooling step. This workflow was optimized to enable a complete walkaway solution with reliable performance. The NEXTFLEX® Human CES Panel incorporates enzymatic fragmentation, bead-based concentration, and a normalization process, eliminating the need for mechanical fragmentation vacuum concentrator, devices to characterize final libraries.