Coverage of 24 Hotspots for Cardiovascular Disease
The NEXTFLEX® Cardiovascular Disease Amplicon Panel contains primer pairs and reagents needed to amplify 24 hotspots for cardiovascular disease from DNA isolated from fresh or frozen material, and prepare libraries for Illumina® sequencing.
A Complete Solution for Sequencing of Cardiovascular Disease Hotspots
The NEXTFLEX® Cardiovascular Disease Amplicon Panel is a complete, validated solution that targets 24 hotspots for cardiovascular disease using 22 PCR primer pairs. This kit requires only 20 ng of DNA isolated from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.
The NEXTFLEX® Cardiovascular Disease Amplicon Pane has the capability of multiplexing up to 1,700 samples at 100x coverage and 90 samples at 2000x coverage for detection of germline mutations on a single Illumina® 2×300 MiSeq® lane.