Complete Amplicon Panel Library Prep Kits that Deliver on Performance
As next-gen sequencing (NGS) becomes more and more commonplace due to its unrivaled multiplexing capabilities and potential for molecular discovery, the power of targeted sequencing must be highlighted for its applicability in almost any field of basic, clinical, or translational research. Amplicon panels are the technology of choice for small target panels, typically under 50kb of sequence. No doubt, they are also the least expensive and most versatile option for sequencing, as fewer reads are required and different strategies can be employed to tackle the resolution of difficult genomic regions, such as those that are highly AT-rich, GC-rich, or possess paralogues or pseudogenes that may be masking the actual gene of interest. Proper amplicon panel design and tactics are needed to ensure high coverage, uniformity, and specificity to assure complete coverage of the sequence without gaps in the intended target regions, as well as to minimize off-target reads, which can be a problem in other sequencing approaches.
As amplicon sequencing is based on ultra-deep sequencing of PCR products for analyzing genetic variations, the technical design and performance of an amplicon-based assay must allow researchers to focus on the interrogation of key genomic regions of interest using a simple workflow and dependable chemistry. The NEXTFLEX® amplicon panels allow researchers to sequence large numbers of targeted gene regions for the analysis of mutational hot spots within a subset of genes, and gain insight into copy number variations (CNVs), well-defined gene fusions, SNPs or indels.
NEXTFLEX® amplicon panel technology boasts high levels of uniformity with no amplicon drop outs, essentially creating a gap guard for your target region which is verified by sequencing. Primers are designed to have high levels of specificity and minimize reads lost to non-alignment that may be caused by primer dimers, adapter dimers, or unaligned low quality and/or chimeric reads. High coverage is attained even with a low number of reads, thereby allowing high level multiplexing or the use of a smaller sequencing cartridge. The NEXTFLEX® amplicon panels are easily scalable, simple to use, fast, and cost-effective kits which can be applied to a broad range of genes.
The pre-designed NEXTFLEX® amplicon panels cater to the needs of researchers investigating cancer & cancer predisposition, newborn syndromes, and inherited disorders using low inputs of gDNA. All pre-designed panels have been performance-verified by Illumina® Miseq® sequencing, but are easily portable to Thermo Fisher® Scientific Ion Torrent™ sequencing. Please inquire for additional details.
Furthermore, the NEXTFLEX® amplicon panel technology can be optimized for researchers interested in custom panel design and collaboration. The contact form can be found below.
Kits are comprised of target-specific primer pools, library prep reagents, including beads, and barcodes for up to 384 samples for Illumina® sequencing. The kit contains enough material to prepare 8, 48, or 96 samples and has a shelf life of 12 months when stored properly.