Automation for Library Prep for Target Capture
The NEXTFLEX® Rapid Pre-Capture Combo Kit is compatible with Beckman Biomek® FX and Biomek® FXP Laboratory Automation Workstations. A program is currently being validated for use with the Sciclone® NGS, Sciclone® NGSx and Sciclone® G3 workstations. For more information contact [email protected].
Multiplex up to 96 Samples with SeqCap® Target Capture
The NEXTFLEX® DNA Barcodes and NEXTFLEX® DNA Barcode Blockers for SeqCap® are included in the NEXTFLEX® Rapid Pre-Capture Combo Kit and are also available separately. Our white paper written in conjunction with Roche®, An Integrated Solution to Simplify Library Preparation and Multiplexing for NimbleGen® Sequence Capture, demonstrates the efficacy of this kit when used with the NimbleGen® SeqCap® EZ Library system for library prep in preparation for Illumina® sequencing.
Achieving High Coverage & Yield from GC & AT Rich Genomes
The PCR amplification step in library preparation introduces biases including uneven coverage of regions with extreme base composition, increased numbers of duplicate fragments, decreased mapping quality and poor variant calling. In our white paper, Achieving High Coverage and Yield from GC and AT Rich Genomes, we show that the NEXTFLEX® PCR polymerase contains a highly optimized and robust enzyme that exhibits minimal GC bias and produces uniform coverage of difficult to sequence genomes.