Whole Genome Sequencing (WGS) is neither time nor cost effective for the identification and analysis of specific genomic variants. Target capture strategies address this by reducing genome complexity to what is relevant to the question being asked.
Target capture approaches require whole genome library preparation followed by systematic target selection through probe hybridization to genomic regions of interest followed by sequencing. Pre-capture pooling of multiple samples prior to target capture is a cost effective method to maximize the amount of data obtained for multiple samples in a single sequencing run. NEXTFLEX® index specific blocking oligos.
We offer complete NEXTFLEX® library prep kits, adapters and blockers for Illumina® sequencing with the ability to multiplex up to 96 samples which are optimized for use with target enrichment systems including Agilent® SureSelect® XT2® systems and the Roche NimbleGen® SeqCap® EZ Library target enrichment system. Additionally, library prep kits, barcodes, and barcode blockers may be purchased separately for other solution-based hybridization target capture approaches.
Please contact us at [email protected] to discuss available protocols and methods.