Targeted sequencing, which allows you to focus on sequences and region of interest, encapsulates two different techniques: a broad, target capture via a hybridization method or a more focused, PCR-based amplicon panel design. Among its most notable benefits compared to other methods in NGS such as WGS or WES, is the fact that because less genomic sequence is being interrogated, only target regions of interest are sequenced. This means that by focusing the sequencing real estate of the flow cell to only regions of interest, genomic sequences can be enriched anywhere from 100 to a 1 million times compared to DNA-sequencing. This is critical for some applications where the typical 10x or 100x coverage obtained by DNA-sequencing or even large exome-sequencing may not be sufficient, such as for the detection of low level somatic variants that may require 3,000x coverage or more to be detected at a frequency of 5% or less. Similarly, great confidence can be got with deep sequencing via NGS than Sanger sequencing for these low-frequency variant calls.
PerkinElmer offers a complete line of NEXTFLEX® library preparation kits to meet your targeted sequencing needs.