TARGETED SEQUENCING
For Illumina® Platforms
Targeted Sequencing
Targeted sequencing, which allows you to focus on sequences and region of interest, encapsulates two different techniques: a broad, target capture via a hybridization method or a PCR-based amplicon panel design. Among its most notable benefits of targeted sequencing compared to other methods in NGS such as WGS or WES, is the fact that because less genomic sequence is being interrogated, only target regions of interest are sequenced. This means that by focusing the sequencing real estate of the flow cell to only regions of interest, genomic sequences can be enriched anywhere from 100 to a 1 million times compared to DNA-sequencing. This is critical for some applications where the typical 10x or 100x coverage obtained by DNA-sequencing or even large exome-sequencing may not be sufficient, such as for the detection of low level somatic variants that may require 3,000x coverage or more to be detected at a frequency of 5% or less. Similarly, great confidence can be got with deep sequencing via NGS than Sanger sequencing for these low-frequency variant calls.
Revvity offers a complete line of NEXTFLEX® library preparation kits to meet your targeted sequencing needs.
Exome Panels
Centered around the NEXTFLEX® Human Whole Exome Sequencing Panel and NEXTFLEX® Core Exome Sequencing v2 panel, PerkinElmer’s standardized and tested targeted capture workflows ensure time and cost savings to labs that are looking to establish genetic analysis or expand their current test menu.
16S & 18S Sequencing Kits
16S and 18S rRNA sequencing are useful and cost-effective NGS tools for microbial sequencing for metagenomic studies comparing different species of bacteria or for eukaryotic cells (18S ITS) present in a given sample without the need for cultures. 16s rRNA is a widely used gene marker for genus and species identification in bacteria and archaea for its minuscule rate of substitution in the highly conserved sequences outside of the hypervariable regions. 18S rRNA is routinely investigated in the phylogenetic study of fungi as it contains more hypervariable domains than 16S. The speed and economy associated with amplicon sequencing is maximized with this approach catered to population genetics and microbial profiling for interrogating the phylogeny and taxonomy of samples from complex microbiomes.
NEXTFLEX® Variant-Seq™ SARS-CoV-2 Kit
The NEXTFLEX® Variant-Seq™ SARS-CoV-2 kit has optimized the identification and tracking of SARS-CoV-2 variants by quickly, easily, and affordably identifying all mutations in a SARS-CoV-2 sample using amplicon sequencing. Sample preparation has been simplified, throughput has been increased, and multiple automation solutions offer flexibility based on your lab’s needs.