IMPROVE YOUR miRNA DISCOVERY BY BLOCKING tRNA/YRNA FRAGMENTS

NEXTFLEX® tRNA/YRNA Blockers for Small RNA-seq Library Prep

Blockers

  • NOVA-513121

       $33

    8 RXNS
Blockers

  • NOVA-513122

       $200

    48 RXNS
Blockers

  • NOVA-513123

       $399

    96 RXNS

Increase miRNA sequencing reads NEXTFLEX® tRNA/YRNA blockers into your small RNA-sequencing workflow. These blockers reduce the number of YRNA and tRNA fragments in small RNA libraries, increasing the miRNA reads. tRNA and YRNA fragments are abundant in sample types like plasma and serum. For researchers who are primarily interested in miRNAs and other small RNAs, reads lost to the abundant tRNA and YRNA fragments leads to a lower number of reads mapping to their RNA species of interest. Coupling the NEXTFLEX® tRNA/YRNA blockers with the NEXTFLEX® small RNA sequencing chemistry and data security of UDIs allows users to maximize the patented and patent-pending technology for ligase-associated bias reduction, which enables superior miRNA discovery for the same sequencing depth.

Reducing Reads from tRNA and YRNA in Plasma

The addition of the NEXTFLEX® tRNA/YRNA blockers reduce the relative number of YRNA and tRNA reads in sequencing libraries and increase the relative number of reads mapping to miRNAs. This reduces sequencing costs and increases sequencing depth, increasing the efficiency of biomarker discovery.

tRNA YRNA

Blocking efficiency of the NEXTFLEX® tRNA/YRNA blockers. Libraries were prepared using the NEXTFLEX® small RNA-seq kit v3. This was done using cfRNA isolated from 600 μL of commercially available plasma that had either high tRNA/YRNA abundance or low tRNA/YRNA abundance using a modified protocol on the Zephyr® G3 NGS workstation. Libraries were sequenced on the Illumina® MiSeq® instrument, and results were analyzed for % alignment to tRNA/YRNA using a proprietary script.

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For research use only. Not for use in diagnostic procedures.