IMPROVE YOUR miRNA DISCOVERY BY BLOCKING tRNA/YRNA FRAGMENTS
NEXTFLEX® tRNA/YRNA Blockers for Small RNA-seq Library Prep
- Reduce the number of YRNA and tRNA fragments in small RNA libraries
- Increase the miRNA reads
- Reduce sequencing costs
- Increase sequencing depth
- Increase the efficiency of biomarker discovery
- Tested with NEXTFLEX® Small RNA-seq kit V3 and NEXTFLEX Small RNA-seq kit v3 with UDIs
NOVA-513121
8 RXNS
NOVA-513122
48 RXNS
NOVA-513123
96 RXNS
Increase miRNA sequencing reads NEXTFLEX® tRNA/YRNA blockers into your small RNA-sequencing workflow. These blockers reduce the number of YRNA and tRNA fragments in small RNA libraries, increasing the miRNA reads. tRNA and YRNA fragments are abundant in sample types like plasma and serum. For researchers who are primarily interested in miRNAs and other small RNAs, reads lost to the abundant tRNA and YRNA fragments leads to a lower number of reads mapping to their RNA species of interest. Coupling the NEXTFLEX® tRNA/YRNA blockers with the NEXTFLEX® small RNA sequencing chemistry and data security of UDIs allows users to maximize the patented and patent-pending technology for ligase-associated bias reduction, which enables superior miRNA discovery for the same sequencing depth.
Reducing Reads from tRNA and YRNA in Plasma
The addition of the NEXTFLEX® tRNA/YRNA blockers reduce the relative number of YRNA and tRNA reads in sequencing libraries and increase the relative number of reads mapping to miRNAs. This reduces sequencing costs and increases sequencing depth, increasing the efficiency of biomarker discovery.
Blocking efficiency of the NEXTFLEX® tRNA/YRNA blockers. Libraries were prepared using the NEXTFLEX® small RNA-seq kit v3. This was done using cfRNA isolated from 600 μL of commercially available plasma that had either high tRNA/YRNA abundance or low tRNA/YRNA abundance using a modified protocol on the Zephyr® G3 NGS workstation. Libraries were sequenced on the Illumina® MiSeq® instrument, and results were analyzed for % alignment to tRNA/YRNA using a proprietary script.