A recent study published in Veterinary Clinical Pathology by Chu, C.P., & Nabity, M.B., provided a comprehensive assessment of the optimal miRNA isolation and library preparation methods for small RNA‐seq research using canine biofluids. For library prep analysis, they compared the NEXTFLEX® Small RNA-seq kit v3 and the Illumina® TruSeq® Small RNA-seq kit.
The authors concluded that the NEXTFLEX® Small RNA-seq kit v3 is the optimal library preparation method for detection of potential biomarkers in canine serum and urine. Additionally, the library preparation method used to identify biomarkers in biofluids introduced a much higher variation in miRNA expression than the miRNA isolation method did. Using urine samples, the NEXTFLEX® Small RNA-seq kit v3 was the only kit that successfully generated libraries.
Using serum samples, NEXTFLEX® Small RNA-seq kit v3 also excelled and produced:
- More total reads
- Higher genome mapping rates
Read the Research:
Chu, C. P., & Nabity, M. B. (2019). Comparison of RNA isolation and library preparation methods for small RNA sequencing of canine biofluids. Veterinary Clinical Pathology. doi:10.1111/vcp.12743