Gel-Free Small RNA Library Prep with Reduced Bias
The NEXTFLEX® Small RNA-Seq Kit v4 uses patent-pending technology to provide a completely gel-free small RNA library preparation solution for Illumina® sequencing platforms. Our approach to reducing bias involves optimization of the ligation reaction and the use of dimer reduction oligonucleotides. These changes results in decreased bias in comparison to NEXTFLEX® Small RNA-Seq Kit v3 and standard protocols.
The legacy NEXTFLEX Small RNA-seq kits v3 are still available and can be found here.
NEXTFLEX® Small RNA-Seq Kit v4 allows preparation of libraries ready for sequencing with a simplified workflow in approximately 5 hours, with as little input as 1 ng of total RNA. Libraries prepared with this kit have a higher proportion of mapping reads and unique miRNAs than leading competitors (Figure 1 and 2).