Small RNA Library Prep with Randomized Adapters for Reduced Sequencing Bias and Unique Dual Indexes to Mitigate Index Hopping
The NEXTFLEX® small RNA-seq v3 automation kit with UDIs uses the same popular chemistry of the NEXTFLEX small RNA-seq kit v3, which incorporates technologies that reduce small RNA ligation bias, increase ligation efficiency, reduce adapter-dimer formation, and build in a new dimension of data quality, integrity, and accuracy with the integration of Unique Dual Indexes (UDIs) for Illumina® sequencing on the MiSeq®, HiSeq® 2000/2500, and NovaSeq® platforms. The addition of UDI barcodes for small RNA sequencing allows confident multiplexing of up to 192 samples, all while mitigating the risk of index hopping and spread of signal that can occur on a patterned flow cell. The purity of each index sequence is validated by sequencing for high confidence in the resulting sequencing data quality.
The NEXTFLEX® small RNA-seq v3 automation kit with UDIs is compatible with cell-free RNA, such as RNA isolated from plasma. Users who wish to deplete the abundant tRNA fragments and Y RNA fragments found in many types of cell-free RNA should use the NEXTFLEX® tRNA/YRNA blocker (not included in kit). If NEXTFLEX® tRNA/YRNA blockers are of interest, please contact us at [email protected]