Figure 1. NEXTFLEX® Combo-Seq mRNA/miRNA kit discovers ~60% more transcripts compared to Competitor N’s mRNA-seq workflow. Libraries were generated from 20 ng of Biochain Human Universal RNA (R4234565) using the NEXTFLEX® Combo-Seq mRNA/miRNA Kit and Competitor N’s directional mRNA-seq workflow. Sequencing libraries were downsampled to 10M reads and processed using the exceRpt pipeline (https://hub.docker.com/r/rkitchen/excerpt). Pipeline output files were used to enumerate ”transcripts discovered.” Transcripts were only marked “discovered” if >= 3 reads mapped to a given transcript and if each read aligned in the appropriate direction (sense for the NEXTFLEX® kit and antisense for Competitor N’s kit). For “long RNA,” transcripts were counted using the “geneLevel” read counts files. In the figure, pink indicates transcripts which were detected by both workflows, whereas purple and red refer to NEXTFLEX® and Competitor N, respectively.