Custom AIR Adenylated Linkers

  • Ideal for next-generation sequencing, miRNA cloning and miRNA sequencing
  • 3’ end blocks prevent self-ligation
  • Adenylated 5′ end reacts with T4 RNL2 Ligase, truncated
  • Phosphorothioate and other modifications available
  • Predesigned linkers are also available
  • You can create your own miRNA barcodes
Technical Support
For research use only. Not for use in diagnostic procedures.

Design Your Own miRNA Cloning Linkers

To request a quote for custom adenylated adapters contact us at [email protected].

The AIR Adenylated Linkers are 5’adenylated oligos with 3’end blocks, which are ready for use in NGS or miRNA cloning. Custom adaptors can be designed for use with any NGS platform. AIR Adenylated Linkers are activated adenylated oligos that preclude the need for ATP in RNA Ligase 2 (RNL2) reactions. This allows for specific and clean ligations.

Selected Publications that Cite the Use of Custom AIR Adenylated Linkers:

  • Alekseyenko, A. A., et al. (2015) UNIT 21.30 BioTAP-XL: Cross-linking/Tandem Affinity Purification to Study DNA Targets, RNA, and Protein Components of Chromatin-Associated Complexes. Current Protocols in Molecular Biology. doi: 10.1002/0471142727.mb2130s109.
  • Benes, V., et al. (2015) Identification of cytokine-induced modulation of microRNA expression and secretion as measured by a novel microRNA specific qPCR assay. Scientific Reports. 5:11590. doi:10.1038/srep11590.
  • Haenni, S. et al. (March 2012) Analysis of C. elegans intestinal gene expression and polyadenylation by fluorescence-activated nuclei sorting and 3′-end-seq. Nuc Acid Res, 40:6
  • Available in 1 nm to 1 µm quantities
  • Most custom AIR Adenylated Linkers have a 10 day turnaround time.
  • Validated on an Illumina® sequencing platform
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