For Illumina® Platforms

Reducing Small RNA Ligation Bias – Small RNA Library Prep Solutions for Illumina® Sequencers

Small RNA sequencing is ideal for quantitative profiling of small RNAs (microRNAs, piRNAs and snoRNAs).  It is used to analyze tissue specific miRNA expression patterns, disease associations and to discover previously uncharacterized miRNAs. However, ligation specific biases reduce the ability of small RNA-seq to accurately quantify microRNA. These biases are mitigated through the incorporation of adapters with degenerate bases during library preparation.

PerkinElmer’s line of NEXTFLEX® Small RNA Kits for Illumina® sequencing are ideal for the conversion of these small RNA transcripts into barcoded cDNA libraries for next-generation sequencing on Illumina® sequencing platforms. With the incorporation of randomized adapters to reduce bias and optimized ligation efficiency; the NEXTFLEX® Small RNA kit v3  offers better data quality and a greater proportion of productive sequence reads than competitive small RNA library prep protocols.

  • Gel-free Small RNA Library Prep Kits with Improved Discovery Rates

    • NEXTFLEX® Small RNA-Seq Kit v4 – Simplified miRNA low input workflow delivering improved discovery rates. Gel-free from as little as 1 ng of total RNA
  • Small RNA-Seq Kit

    • NEXTFLEX® Small RNA-seq v3 Kit – incorporates technologies to reduce small RNA ligation bias, increase ligation efficiency, reduce adapter-dimer formation. Versions include UDIs.
  • Small RNA and mRNA Library Prep in a Single Workflow

  • Increase mRNA Reads in Small RNA-seq Workflows

Contact us reduce your small RNA-seq bias

For research use only. Not for use in diagnostic procedures.