Optimized to Improve Data Integrity
The NEXTFLEX® Unique Dual Index barcodes contain two unique indexes on each adapter, with a hamming distance of at least three throughout the entire series, to allow error correction. The NEXTFLEX® Unique Dual Index RNA-seq barcodes can be used for PCR-free RNA sequencing applications. Patterned flow cells found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity. Every lot is functionally validated and tested for index purity by sequencing.