NEXTFLEX® RNA-Seq 2.0 Unique Dual Index Adapters (Set of 1,536)

  • 1,536 RNA-seq UDI barcodes for super high multiplexing capacity with paired-end or single-read sequencing
  • Every lot is functionally validated for index purity by sequencing
  • Ideal for Illumina® NovaSeq® runs or projects
  • UDI adapters with 10 nt indexes
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  • NOVA-524100

    BARCODES 1-1,536
    3,072 rxns
For research use only. Not for use in diagnostic procedures.

Ultra High Sample Multiplexing Options for RNA-Seq on Illumina® Sequencers

This set of 1,536 NEXTFLEX® RNA-Seq 2.0 UDI barcodes (10-nt index) are plated at 6.25 µM stock concentration to be used with the NEXTFLEX® Rapid Directional RNA-Seq kit 2.0, CRISPRclean® Plus Stranded Total RNA Prep with rRNA Depletion, or other RNA-Seq library preparation kits which ligates adapters for multiplexing on Illumina® sequencer to adenylated fragments using similar adapter concentrations.

A set of up to 384 NEXTFLEX® RNA-seq 2.0 UDI barcodes are also available for labs with lower throughput needs.

Reliably Mitigate Index Hopping for Improved Data Integrity

Designed with a hamming distance of at least three throughout the entire series that allows for error correction, the NEXTFLEX® Unique Dual Index barcodes contain two unique indexes on each adapter. These NEXTFLEX® Unique Dual Index RNA-seq 2.0 barcodes can be used for PCR-free RNA sequencing applications as well. If using the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms that utilize patterned flow cells, these barcodes would be useful to counter the known increased rates of sample mis-assignment that is thought to occur during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity. Every lot is tested for index purity by sequencing.


  • NEXTFLEX® Unique Dual Index Barcodes* (6.25 µM)
  • NEXTFLEX® Primer Mix** (50 µM)

The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc).

* These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.

** The Primer Mix 2.0 is only intended for use with the NEXTFLEX® Rapid RNA-seq kit 2.0; if the primer mix is to be used with any other library kits of choice, additional dilutions may be required. For additional guidance, please inquire at [email protected].

For availability of single use plates for automation, please inquire for additional details.


  • Sequences of NEXTFLEX® RNA-seq 2.0 Unique Dual-Indexed Barcode (set of 1,536) Indexes – Excel


Each NEXTFLEX® RNA-seq 2.0 Unique Dual Index barcodes (1-1,536) contains 1,536 unique dual-index barcodes, enabling users to multiplex up to 1,536 libraries per flow cell lane. Each lot of the NEXTFLEX® RNA-seq 2.0 Unique Dual Index barcodes (1-1,536) is functionally validated and tested for index purity by sequencing. NEXTFLEX® RNA-seq 2.0 Unique Dual Index barcodes are supplied in 96-well plates with each well containing 2 reactions of each barcode. These kits ship on dry ice. The shelf life of each reagent is at least one year when stored properly.

For automation single-use plates, NEXTFLEX® Unique Dual Index barcodes (1-1,536) may be at a different specified concentration and each well will contain 1 reaction of each barcode.  Please inquire for additional details. These kits ship on dry ice.

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