NEXTFLEX® Unique Dual Index Barcodes

  • For multiplexing sequencing libraries compatible with both paired-end and single-read Illumina® sequencing
  • Every lot is functionally validated and tested for index purity by sequencing
  • Up to 1,536 adapters available for purchase off-the-shelf

NEXTFLEX® UDI Barcodes Offer the Best-in-Class Sample Multiplexing Options for Illumina® Sequencing

The NEXTFLEX® Unique Dual Index barcodes allow a broad range of multiplexing – users can multiplex anywhere between two libraries to 1,536 libraries in a single sequencing run. The wide range of multiplexing options enables barcode rotation (avoiding frequent use of the same barcode set) to mitigate run-to-run sample carryover and crosstalk. NEXTFLEX® Unique Dual Index Barcodes are compatible with NEXTFLEX® library prep kits designed for Illumina® paired-end and single-read sequencing and other workflows for Illumina® sequencing that involves the ligation of adapters to adenylated fragments.

NEXTFLEX® UDI Barcodes Improve Data Integrity

The NEXTFLEX® Unique Dual Index barcodes utilize two unique indexes on each UDI adapter, with a hamming distance of at least three throughout the entire series to permit error correction. Each NEXTFLEX® Unique Dual Index Barcode comprises the entire flow cell binding site, sequencing primer binding site and index sequences, thereby making PCR enrichment optional. Patterned flow cells, such as those found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms, have been known to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity. Every lot is functionally validated and tested for index purity by sequencing.

Reduce Run-to-Run Sample Carryover & Sample Crosstalk with UDIs

Even users who don’t routinely perform high-throughput multiplexing benefit from the superior design of the NEXTFLEX® Unique Dual Index Barcodes. The ability to avoid using the same set of indices consecutively reduces concerns about run-to-run sample carryover and sample crosstalk. They are designed to specifically address the index hopping, or spread of signal, phenomenon associated with Illumina® platforms that utilize a patterned flow cell.

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For research use only. Not for use in diagnostic procedures.