NEXTFLEX® UDI Barcodes Improve Data Integrity
The NEXTFLEX® Unique Dual Index barcodes utilize two unique indexes on each UDI adapter, with a hamming distance of at least three throughout the entire series to permit error correction. Each NEXTFLEX® Unique Dual Index Barcode comprises the entire flow cell binding site, sequencing primer binding site and index sequences, thereby making PCR enrichment optional. Patterned flow cells, such as those found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms, have been known to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity. Every lot is functionally validated and tested for index purity by sequencing.