- NEXTFLEX-HT™ Barcode Adapter (25 µM)
- NEXTFLEX® Primer Mix (12.5 µM)
- Sequences of NEXTFLEX-HT™ Barcode Indexes – Excel / PDF
The NEXTFLEX-HT™ Barcodes Kits contain 6 or 96 unique single-index barcodes, enabling users to multiplex up to 384 samples per flow cell lane. NEXTFLEX-HT™ Barcodes – 6 are supplied in tubes containing 8 reactions worth of each barcode. The sets of 96 NEXTFLEX-HT™ Barcodes are supplied in 96-well plates with each well containing 2 reactions of each barcode. These kits ship on dry ice.
What is the concentration of the NEXTFLEX-HT™ Barcodes?
The concentration of the NEXTFLEX-HT™ Barcodes is 25 µM.
Are these barcodes compatible with both paired-end and single-read Illumina® sequencing?
Yes, the NEXTFLEX-HT™ Barcodes can be used with both SR and PE sequencing.
Do the NEXTFLEX-HT™ Barcodes Kits contain PCR primers?
Yes, these barcodes are supplied with PCR primers.
At what concentration are the NEXTFLEX® PCR primers supplied?
The NEXTFLEX® PCR Primers concentration is 12.5 µM.
Can I order more NEXTFLEX® PCR primers alone?
Yes, the NEXTFLEX® PCR Primers are available in sets of 48, 96, 192, 384 or 768 reactions. Contact [email protected] for more information.
How is the quality of the NEXTFLEX-HT™ Barcodes assured?
Each lot of the NEXTFLEX® barcodes is sequence verified on an Illumina® MiSeq® instrument.
What type of purification is used when the NEXTFLEX® DNA Barcodes are synthesized?
In order to ensure the highest quality sequencing data, the NEXTFLEX-HT™ Barcodes are HPLC-purified, minimizing the risk of mispriming events due to truncations and other data quality-compromising errors.
Are the NEXTFLEX-HT™ Barcodes methylated?
No they are not methylated; therefore they are not compatible with bisulfite-seq applications. However, they are compatible with all other DNA-Seq applications including MeDIP and MeCAP sequencing. The NEXTFLEX® Bisulfite-Seq Barcodes are provided for library prep requiring methylated adapters.