Increase Scale while Reducing Cost
The NEXTFLEX-96™ RNA-Seq Barcodes are adapters that were designed for use with the NEXTFLEX® Rapid RNA-Seq Kit and the NEXTFLEX® Rapid Directional RNA-Seq Kit. These barcodes are not recommended for use with the new NEXTFLEX® Rapid Directional RNA-seq kit 2.0.
The NEXTFLEX-96™ RNA-Seq Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTFLEX-96™ RNA-Seq Barcodes kits accomplish this by using an indexed adapter with an 8 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTFLEX® index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. The NEXTFLEX-96™ RNA-Seq Barcode index sequences are designed to have a hamming distance of 4, allowing discrimination of different adapters even in the case of an error in the barcode read. These adapters are supplied a 96-well format.
Do you want to index you samples but don’t want to use 96 different barcodes? We also offer the NEXTFLEX® RNA-Seq Barcodes in sets of 6, 12, 24 and 48 barcodes.
For larger volume requirements, customized and bulk packaging is available. Please contact [email protected] for further information.