Pool Multiple Library Preparations in a Single Flow Cell Lane
The NEXTFLEX-96™ DNA Barcodes are compatible with Illumina® sequencing and provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. These adapters compatible with Illumina® sequencing utilize an indexed adapter with an 8 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads caused by single base errors introduced during PCR. The NEXTFLEX® index is contained within the adapter sequence, eliminating the need to perform PCR to add flow cell binding sequences. The NEXTFLEX-96™ DNA Barcode index sequences are designed to have a hamming distance of 4, allowing discrimination of different barcodes even in the case of an error in the barcode read.
These adapters are supplied in either a 96-well format (cat # NOVA-514105) or in microfuge tubes (cat # NOVA-514106). These barcodes can be used with single, paired-end, and multiplex reads and are compatible with the NEXTFLEX® Rapid DNA-Seq Kit 2.0, the NEXTFLEX® Methyl Sequencing Kit, the NEXTFLEX® Cell Free DNA-Seq Kit and other genomic DNA library prep protocols.
Do you want to index your samples but don’t want to use 96 different barcodes?
We also offer the NEXTFLEX® DNA Barcodes in sets of 6, 12, 24, and 48 barcodes.
Do you want to multiplex more than 96 samples?
The NEXTFLEX-HT™ Barcodes are ideal for researchers who want to multiplex up to 384 samples with single-index adapters.
Do you want to multiplex RNA samples?
NEXTFLEX® RNA-Seq Barcodes and NEXTFLEX-96™ RNA-Seq Barcodes are also available for your RNA-Seq multiplexing needs.