Increase Scale while Reducing Your Cost
The NEXTFLEX-96™ ChIP-Seq Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTFLEX-96™ ChIP-Seq Barcodes kits accomplish this by using an indexed adapter with an 8 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads caused by single base errors introduced during PCR. The NEXTFLEX® index is contained within the adapter sequence, eliminating the need to perform PCR to add flow cell binding sequences. The NEXTFLEX-96™ ChIP-Seq Barcode index sequences are designed to have a hamming distance of 4, allowing discrimination of different barcodes even in the case of an error in the barcode read. These adapters, compatible with Illumina® platforms, are available in custom formats.
Do you want to index your samples but don’t want to use 96 different barcodes? We also offer the NEXTFLEX® ChIP-Seq Barcodes in sets of 6, 12, 24, and 48 barcodes.
These barcodes can be used with single, paired-end, and multiplex reads and are compatible with the NEXTFLEX® Rapid DNA-Seq Kit 2.0 and the NEXTFLEX® ChIP-Seq Kits and other low input DNA-seq protocols.
For larger volume requirements, customized and bulk packaging is available. Please contact [email protected] for further information.