IDEAL FOR ILLUMINA® LIBRARY PREP FOR REDUCED REPRESENTATION BISULFITE SEQUENCING (RRBS) &
WHOLE-GENOME BISULFITE SEQUENCING (WGBS)

NEXTFLEX® Bisulfite Library Prep Kit for Illumina® Sequencing

  • Compatible with total bisulfite sequencing and reduced representation
  • Single nucleotide resolution of methylation sites
  • Uracil insensitive polymerase designed for bisulfite-converted DNA
  • Methylome-level assessment with broad genome coverage
  • Enhanced adapter ligation technology with NEXTFLEX® Ligation
  • Bead-based cleanup
  • Automation-friendly workflow is compatible with liquid handlers
  • Methylated barcoded adapters for multiplexing contain embedded index sequence
  • Functionally validated with Illumina® sequencing platforms

  • NOVA-5119-01

    8 RXNS

  • NOVA-5119-02

    48 RXNS
For research use only. Not for use in diagnostic procedures.

BISULFITE-SEQ ADDITIONAL COMPONENTS


  • NOVA-511911

    BISULFITE-SEQ BARCODES - 6
    48 RXNS

  • NOVA-511912

    BISULFITE-SEQ BARCODES-12
    96 RXNS

  • NOVA-511913

    BISULFITE-SEQ BARCODES - 24
    192 RXNS

RRBS & WGBS Sequencing

The NEXTFLEX® Bisulfite-Seq Library Prep Kit is designed for both reduced representation bisulfite sequencing (RRBS) and whole-genome bisulfite sequencing (WGBS). It generates single, paired-end and multiplexed DNA libraries for sequencing on Illumina® platforms. The NEXTFLEX® U+ Polymerase Mix included in the kit is able to read through uracil present in DNA templates and has been optimized to amplify bisulfite converted templates.

RRBS Bisulfite-Seq Kit

RRBS: 10 ng of Msp1 digested DNA input, 18 PCR cycles, 10 nm yield

RRBS BIsulfite-Seq Kit

RRBS: 1 ug Msp1 digested DNA input, 18 PCR cycles, 32 nm yield

WGBS Bisulfite-Seq

WGBS: 10 ng of 200 bp sheared DNA input, 18 PCR cycles, 58 nm yield

WGBS Bisulfite-Seq

WGBS: 1 ug 200 bp sheared DNA input, 15 PCR cycles, 28 nm yield

Complete Methylome Analysis

The NEXTFLEX® Bisulfite-Seq Kit is a versatile kit designed to facilitate assessment of the methylation state of the genome and simplify workflow by using master mixed reagents and magnetic bead based cleanup to reduce pipetting and eliminate time-consuming steps in library preparation. In addition, the availability of up to 24 methylated NEXTFLEX® Bisulfite-Seq barcoded adapters makes multiplexing simple. This kit features Enhanced Adapter Ligation Technology, resulting in library preps with a larger number of unique sequencing reads. This specially designed NEXTFLEX® ligation enzymatic mix allows users to perform ligations with longer adapters and superior ligation efficiencies.

Focused Sequence Depth

The NEXTFLEX® Bisulfite-Seq Kit with reduced representation utilizes a restriction enzyme that leads to selective amplification of CpG regions, resulting in focused sequence depth when used with an MSP1 enzyme. The NEXTFLEX® Bisulfite-Seq Kit can also be used without the MSP 1 Restriction Enzyme for genome-wide methyl-seq analysis, including under-represented CpG regions.