SIMPLIFIED DNA-SEQ WORKFLOW – FULLY AUTOMATED NORMALIZATION AND FRAGMENTATION

NEXTFLEX® Rapid XP V2 DNA-Seq Kit for Illumina® Platforms

The new NEXTFLEX® Rapid XP V2 DNA-seq kit includes proprietary NEXTFLEX® normalization beads, which provide a consistent mass and sequencing cluster density for all samples in a library pool. This shortens the time needed for quantification and pooling preparation for sequencing in a high throughput lab by up to 3 hours per 96 samples.

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Streamlined DNA-seq library prep workflow

V2

  • NOVA-5149-21

    8 RXNS
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V2

  • NOVA-5149-22

    48 RXNS
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V2

  • NOVA-5149-23

    96 RXNS
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Highly Efficient Ligation with Low Adapter Formation

The NEXTFLEX® Rapid XP V2 DNA-seq kit offers a streamlined workflow with highly efficient ligation, generating low amount of adapter dimers even at low input concentrations.

reduced dimer formation DNA-seq libraries
Figure 1. Low dimer formation with NEXTFLEX® Rapid XP V2 DNA-Seq kit. Different library replicates were prepared using either 500 pg (A) or 1 ng (B) of high-quality genomic DNA as input. Adapter dimer should appear as a peak at ~150 bp.
Reduced GC bias with DNA-seq libraries
Figure 2. Analysis of GC bias from libraries obtained with NEXTFLEX Rapid XP V2 DNA-Seq kit. Results show minimal variability in normalized coverage across 20-60% GC content encompassing ~95% of the human genome (Right: red bars). These are consistent across 1-750ng of genomic DNA input.

Simplify Library Construction with NEXTFLEX® Normalization Beads

Simplified DNA-seq workflow with normalization beads

Flexible Multiplexing Options with 1,536 Barcodes Available for High-Throughput Multiplexing

Barcodes are not supplied with the NEXTFLEX® Rapid XP DNA-seq kit v2 and need to be purchased separately. We offer 384 color-balanced NEXTFLEX® UDI adapters that improve multiplexing capabilities for both low-level and high-level multiplexing needs. Additionally, a set of 1,536 NEXTFLEX® UDI adapters which are compatible with the NEXTFLEX® Rapid XP DNA-seq kit v2 are now available for ultra high-throughput applications.

For more information about barcode options compatible with the NEXTFLEX® Rapid XP DNA-seq kit v2 and bundled pricing, please contact [email protected].

KIT CONTENTS

  • NEXTFLEX® Fragmentation Buffer V2
  • NEXTFLEX® Fragmentation Enzyme V2
  • NEXTFLEX® Ligation Master Mix V2
  • NEXTFLEX® PCR Master Mix V2
  • NEXTFLEX® Primer Mix V2
  • Nuclease-free Water
  • Elution Buffer
  • NEXTFLEX® Cleanup Beads XP
  • NEXTFLEX® Normalization Beads V2

REQUIRED MATERIALS NOT PROVIDED

  • 100 pg – 1 µg of DNA in up to 17.5 µL nuclease-free water.
  • NEXTFLEX® barcodes
  • Ethanol 100% (room temperature)
  • 96 well PCR Plate Non-skirted (Phenix® Research, Cat # MPS-499) or similar
  • 96 well Library Storage and Pooling Plate (Thermo Fisher® Scientific, Cat # AB-0765) or similar
  • Adhesive PCR Plate Seal (Bio-Rad®, Cat # MSB1001)
  • Magnetic Stand -96 (Thermo Fisher® Scientific, Cat # AM10027) or similar
  • Thermocycler
  • 2, 10, 20, 200 and 1000 µL pipettes / multichannel pipettes
  • Nuclease-free barrier pipette tips
  • Vortex
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