Ligation of Adapters to SARS-CoV-2 Amplicon Pools
After generating amplicon pools with SARS-CoV-2 specific primers, you need to add adapters to them to create amplicon libraries for sequencing. While there are many options available, the ability to multiplex up to 1,536 samples simultaneously greatly reduces the both the cost and sequencing time when compared to options that can only multiplex 384 samples at a time.
If your SARS-CoV-2 primer sets for amplicon sequencing generate short tiled amplicons which don’t require fragmentation, we recommend using the NEXTFLEX® Rapid DNA-seq kit 2.0 with the NEXTFLEX® UDI barcodes. This solution allows researchers to multiplex up to 1,536 samples per run, increasing throughput and reducing the cost and time required for sequencing.
If you are using long cDNA which requires fragmentation prior to sequencing, use the NEXTFLEX® Rapid XP DNA-seq kit.