IDEAL FOR SEQUENCING OF cfDNA & ctDNA ON AN ILLUMINA® SEQUENCING PLATFORM

NEXTFLEX® Cell Free DNA-Seq Library Prep Kit 2.0 for Illumina® Sequencing Platforms

  • Designed for low sample input – 10 ng of input cfDNA required
  • Accelerated workflow with minimal hands-on time
  • Flexible adapter barcode options – up to 1,536 unique dual index barcodes are available
  • Compatible with Illumina® sequencing platforms
  • Protocols available for automation on the Sciclone® G3 NGSx, NGSx iQ™ and Zephyr® G3 NGS workstations

  • NOVA-5150-01

    8 RXNS

  • NOVA-5150-02

    48 RXNS
For research use only. Not for use in diagnostic procedures.

Prepare Libraries in Only 2 Hours

The NEXTFLEX® Cell Free DNA-Seq Kit 2.0 can produce libraries from 10 ng of cell free DNA for circulating tumor DNA (ctDNA) or cell free fetal DNA (cffDNA) analysis, in two hours. This low-input library preparation kit delivers high coverage quality and reduced bias for Illumina® sequencing applications. The NEXTFLEX® Cell Free DNA-Seq Kit 2.0 can be used to prepare single, paired-end and multiplexed DNA libraries for sequencing using Illumina®platforms. The availability of up to 1,536 unique dual index barcodes facilitates high-throughput applications without compromising data integrity.

We recommend using the NextPrep-Mag cfDNA Automated Isolation Kit for isolation of circulating cfDNA from blood plasma prior to library prep with the NEXTFLEX® Cell Free DNA-Seq Kit 2.0.

Reduced Bias and Coverage Gaps

NEXTFLEX® Cell Free DNA-Seq Kit 2.0 exhibits minimal GC bias and produces uniform coverage of difficult to sequence genome regions. This kit also offers robust ligation efficiency, resulting in greater library diversity and a larger number of unique sequencing reads. These optimizations enable investigators to obtain more information from each sequencing run.

Flexible Multiplexing Options

The NEXTFLEX® Cell Free DNA-Seq Library Prep Kit 2.0 for Illumina®  sequencing platforms is designed to be used with the NEXTFLEX® Unique Dual Index barcodes (384 barcodes) or the NEXTFLEX® Unique Dual Index barcodes (1,536 barcodes). Thesed NEXTFLEX® Adapters are long, annealed adapters that offer an improved multiplexing workflow and flexible setup.

Automation Solutions

This kit is automated on the Sciclone® G3 NGSx, NGSx iQ™ and Zephyr® G3 NGS workstations. Contact us at [email protected] for more details.