Rapid Cell Free DNA Library Prep for Sequencing on Ion Torrent™ Platforms
The NEXTFLEX® Cell Free DNA Sequencing Kit for Ion Torrent™ platforms is designed to prepare genomic DNA-Seq NGS libraries from 1 – 5 ng of cell free DNA for sequencing using the, Ion S5™, Ion S5™ XL, Ion Torrent™ PGM, and Ion Proton™ sequencing platforms.
The NEXTFLEX® Cell Free DNA Sequencing Kit simplifies workflow by using master mixed reagents and magnetic bead based cleanup, reducing pipetting and eliminating time-consuming steps in library preparation. In addition, the availability of up to 64 unique adapter barcodes enables high-throughput applications.
There are five main steps involved in preparing genomic DNA for Ion Torrent sequencing: DNA extraction, DNA end repair, size selection (optional), adapter ligation and PCR amplification. This kit contains the necessary material to prepare the user’s purified DNA for emulsion PCR and subsequent sequencing. We recommend using the NextPrep-Mag™ cfDNA Isolation Kit for isolation of circulating DNA from blood plasma prior to library prep.
Cell free DNA has become a powerful marker in clinical applications due to the unique origin of DNA molecules present in plasma. Detection of fetal DNA from maternal plasma has proven to be a viable, non-invasive option to identify a variety of fetal traits including: sex determination, sex chromosome-linked disorders and aneuploidy events. Circulating tumor DNA extracted from plasma of symptomatic patients can be used as a non-invasive resource for diagnosis, prognosis, treatment decisions, and follow-up monitoring of cancer patients.