Sequencing of the 18S rRNA Gene
The ITS1 and ITS2 regions within the highly conserved 18S rRNA gene are the gold standards used in eukaryotic molecular phylogeny. These ITS regions contains a high level of sequence variation between closely related fungal species and are easy targets to amplify from minimal quantities of DNA due to the high number of copies found per genome, ranging from 10-200. These are excellent markers for detecting both rare species and those in great abundance within complex communities.
Automate your 18S ITS Library Prep to Increase Throughput and Reduce Errors
A NEXTFLEX® automation protocol is now available on the Sciclone® NGS and NGSx workstations to help labs increase their throughput and reduce human errors. Automated versions of the NEXTFLEX® 18S ITS Amplicon-Seq Library Prep kits are available by request. Please inquire for additional information at [email protected]. Automation kits are custom items and require a minimum of 2 weeks lead time.