VALIDATED 16S V4 rRNA LIBRARY PREP FOR BROAD-RANGE ANALYSIS OF MIXED MICROBIAL POPULATIONS

NEXTFLEX® 16S V4 Amplicon-Seq Kit 2.0 for Illumina® Platforms

  • Fast and easy library prep protocol
  • Low input – Only 1 ng of input required
  • Multiplex up to 384 libraries
  • Custom sequencing primers are not required
  • Automation protocols are now available for the Sciclone® NGS and NGSx Workstations to automate your 16S sequencing
  • Functionally validated with Illumina® sequencing platforms
For research use only. Not for use in diagnostic procedures.

  • NOVA-4203-03

    48 BARCODES
    96 RXNS

  • NOVA-4203-04

    BARCODES 1-96
    192 RXNS

  • NOVA-4203-05

    BARCODES 97-192
    192 RXNS

  • NOVA-4203-06

    BARCODES 193-288
    192 RXNS

  • NOVA-4203-07

    BARCODES 289-384
    192 RXNS

Automated versions of the NEXTFLEX® 16S V4 Amplicon-Seq Library Prep kits 2.0 are available by request. Please inquire for additional information at [email protected].

Ideal for Monitoring Populations of Microbial Communities

The NEXTFLEX® 16S V4 Amplicon-Seq Library Prep Kit 2.0 is designed for the preparation of multiplexed amplicon libraries spanning the V4 hypervariable domain of microbial 16S ribosomal RNA (rRNA) genes. These libraries are compatible with paired-end sequencing on the Illumina® sequencing platforms.

Low Input Requirements for 16S rRNA Sequencing

As little as 1 ng of genomic DNA can be used to generate libraries using the NEXTflex 16S V4 Amplicon-Seq Library Prep Kit 2.0.

Genera measured by analysis of 16S V4 reads

Optimized Protocol Offers Lower PCR Bias and Fewer Off-target Reads

The NEXTFLEX 16S V4 Amplicon-Seq Kit 2.0 incorporates a second PCR step in the protocol for the addition of the sample-specific index which reduces off-target reads encountered during amplicon sequencing.

384 Barcodes Available for Cost-effective 16S V4 rRNA Sequencing

The NEXTFLEX 16S V4 Amplicon-Seq Library Prep Kit 2.0 can be used for multiplexing up to 384 samples, to greatly reduce the cost of sequencing. The barcodes are offered in sets of 4, 12, 48 or 96 barcodes and supplied with 2 reactions worth of each barcode.

Automate your 16S V4 Library Prep to Increase Throughput and Reduce Errors

A NEXTFLEX® automation protocol is now available on the Sciclone® NGS and NGSx workstations to help labs increase their throughput and reduce human errors. Automated versions of the NEXTFLEX® 16S V4 Amplicon-Seq Library Prep kits 2.0 are available by request. Please inquire for additional information at [email protected]. Automation kits are custom items and require a minimum of 2 weeks lead time.