VALIDATED LIBRARY PREP FOR 16S V1-V3 rRNA BACTERIAL METAGENOMICS ANALYSIS

NEXTFLEX® 16S V1-V3 Amplicon-Seq Kit for Illumina® Platforms

  • Optimized protocol offers lower PCR bias and fewer off-target reads
  • Fast library prep protocol
  • Low input – As low as 1 ng of genomic DNA
  • Flexible barcode options– Up to 384 unique barcodes available for multiplexing of libraries
  • Does not require custom sequencing primers
  • Automation protocols are now available for the PerkinElmer Sciclone® NGS and NGSx Workstations to automate your 16S sequencing
  • Functionally validated on the Illumina® MiSeq® sequencer
Gut Microbiome Composition Predicts Infection Risk During Chemotherapy in Children With Acute Lymphoblastic Leukemia
For research use only. Not for use in diagnostic procedures.

  • NOVA-4202-02

    12 BARCODES
    24 RXNS

  • NOVA-4202-03

    48 BARCODES
    96 RXNS

  • NOVA-4202-04

    BARCODES 1-96
    192 RXNS

  • NOVA-4202-05

    BARCODES 97-192
    192 RXNS

  • NOVA-4202-06

    BARCODES 193-288
    192 RXNS

  • NOVA-4202-07

    BARCODES 289-384
    192 RXNS

Automated versions of the NEXTFLEX® 16S V1 – V3 Amplicon-Seq Library Prep kits are available by request. Please inquire for additional information at [email protected].

Preparation of Multiplexed Amplicon Libraries

The NEXTFLEX® 16S V1 – V3 Amplicon-Seq Library Prep Kit is designed for the preparation of multiplexed amplicon libraries that span the hypervariable domains one through three (V1-V3) of microbial 16S ribosomal RNA (rRNA) genes. These libraries are compatible with paired-end sequencing on the Illumina® sequencing platforms.

Fast Library Prep Protocol

There are two main steps involved in 16S V1-V3 amplicon processing: an initial PCR amplification using customized PCR primers that target the V1-V3 domains, and a subsequent PCR amplification that integrates relevant flow cell binding domains and unique 12 base pair sample indices. The limited number of cleanup steps ensures maximum recovery of amplicons for downstream sequencing.

Optimized Protocol Offers Lower PCR Bias and Fewer Off-target Reads

The protocol incorporated in the NEXTFLEX 16S V1 – V3 Amplicon-Seq Kit offers better sequencing results than can be obtained using traditional 16S sequencing protocols. The incorporation of the second PCR step in the protocol for the addition of the sample-specific index reduces the number of off-target reads typically encountered during amplicon sequencing.

Automate your 16S V1 – V3 Library Prep to Increase Throughput and Reduce Errors

A NEXTFLEX® automation protocol is now available on the Sciclone® NGS and NGSx workstations to help labs increase their throughput and reduce human errors. Automated versions of the NEXTFLEX® 16S V1 – V3 Amplicon-Seq Library Prep kits are available by request. Please inquire for additional information at [email protected]. Automation kits are custom items and require a minimum of 2 weeks lead time.