16S ribosomal RNA (rRNA) sequencing is an amplicon sequencing technique used to identify and compare species of bacteria present within a given sample. 16S rRNA gene sequencing is used to study phylogeny and taxonomy of samples from complex microbiomes or environments that are difficult or impossible to study. 16S rRNA sequencing can be used to specify taxonomic assignments, down to the species level. PerkinElmer offers NEXTFLEX® 16S Amplicon-Seq Kits for this purpose, which are optimized library prep kits developed to simplify 16S rRNA sequencing on the Illumina® sequencing platforms. Automation protocols are also available for the PerkinElmer Sciclone® NGS and NGSx Workstations.
Culture-free Identification of Bacteria
The NEXTFLEX 16S Amplicon-Seq Kits enable analysis of the entire microbial community within a sample, without bias introduced by the culturing required for other, non-NGS based, 16S rRNA analysis methods. With the ability to multiplex hundreds of samples in a sequencing run, NGS-based 16S rRNA sequencing is a sensitive and cost-effective technique to identify strains that may not be identified using other methods.
Simplifying 18S rRNA Sequencing for Identification of Fungal and Micro-eukaryotic Species
The NEXTFLEX® 18S ITS Amplicon-Seq Kit is designed to prepare multiplexed amplicon libraries that span the hypervariable Internal Transcribed Spacer (ITS) region of eukaryotic 18S ribosomal RNA (rRNA) genes. 18S rRNA sequencing that captures the ITS1 and/or ITS2 hypervariable Internal Transcribed Spacer regions is recommended for eukaryotic phylogeny investigation of both rare and abundant fungal and micro-eukaryotic species from minimal quantities of DNA in complex communities.
Culture-free Identification of Bacterial and Fungal/micro-eukaryotic Species in a Single Library Prep – NEW!
The NEXTFLEX® 16S V4 & 18S ITS Amplicon-Seq Kit allows the preparation of libraries that cover both the 16S V4 of bacteria and the 18S ITS region of eukaryotic 18S rRNA to allow simultaneous interrogation of bacteria, fungus, and micro-eukaryotes in a single library prep and sequencing run to allow exceptional value to those wanting to identify bacterial and eukaryotic species within complex communities.