16S and ITS Library Prep Kits for Metagenomics Studies

16S ribosomal RNA (rRNA) sequencing is an amplicon sequencing technique used to identify and compare species of bacteria present within a given sample. 16S rRNA gene sequencing is used to study phylogeny and taxonomy of samples from complex microbiomes or environments that are difficult or impossible to study. 16S rRNA sequencing can be used to specify taxonomic assignments, down to the species level. PerkinElmer offers NEXTFLEX^® 16S Amplicon-Seq Kits for this purpose, which are optimized library prep kits developed to simplify 16S rRNA sequencing on the Illumina^® sequencing platforms. Automation protocols are also available for the PerkinElmer Sciclone^® NGS and NGSx Workstations.

Culture-free Identification of Bacteria

The NEXTFLEX 16S Amplicon-Seq Kits enable analysis of the entire microbial community within a sample, without bias introduced by the culturing required for other, non-NGS based, 16S rRNA analysis methods. With the ability to multiplex hundreds of samples in a sequencing run, NGS-based 16S rRNA sequencing is a sensitive and cost-effective technique to identify strains that may not be identified using other methods.

Simplifying the Identification of Fungal and Micro-eukaryotic Species

The NEXTFLEX^® ITS Amplicon-Seq Kit is designed to prepare multiplexed amplicon libraries that span the hypervariable Internal Transcribed Spacer (ITS) region. ITS rRNA sequencing captures the ITS1 and/or ITS2 hypervariable Internal Transcribed Spacer regions. It is recommended for eukaryotic phylogeny investigation of both rare and abundant fungal and micro-eukaryotic species from minimal quantities of DNA in complex communities.