Illumina® & Ion Torrent Sequencing

NGS Library Prep Kits for Illumina® & Ion Torrent Sequencers

High-quality library preparation is a critical step in next-generation sequencing (NGS). PerkinElmer provides a complete portfolio of NGS library preparation kits and barcodes designed to increase the sensitivity, flexibility, and speed of library prep for the Illumina® and Ion Torrent sequencing platforms. We have focused our attention on increasing enzymatic efficiency in our next generation sequencing library prep kits to cater to the customer’s need for a cost effective solution to achieve robust, reproducible, high-quality sequencing results every time.

Completely automated versions of our library prep kits are available for high throughput needs while manual versions cater to the needs of lower throughput users. To meet the demands of high-throughput customers, NEXTFLEX® reagents can also be supplied in bulk quantities and custom formats.

/////  Our library prep kits are optimized to simplify the following applications:

  • NGS Adapters | Barcoded adapters for all of your NGS multiplexing needs

  • Small RNA-Seq  |  Gene silencing and post-transcriptional regulation of gene expression can be investigated by small RNA-seq, which allows for the profiling of all of the small RNA and miRNA present in samples

  • Single Cell RNA-Seq  | Expand the possibilities for your single cell RNA-seq with the HIVE™ scRNAseq Solution, enabling multi-site collection.

  • RNA-Seq  |  Profiling for the analysis of the expression level of RNAs in a given cell or population of cells

  • DNA-Seq  |  Library prep kits for whole genome sequencing and kits

  • Targeted Sequencing  |  Targeted resequencing for a highly targeted approach that analyzes specific genomic regions such as amplicon panels and complete hybrid-capture solutions

  • 16S and 18S rRNA Sequencing  |  16S and 18S ITS rRNA sequencing used to identify and compare bacteria or fungi present within an environmental sample.

  • Whole Genome Amplification  | Amplification of DNA from single cells and other limited DNA templates for NGS and other applications

  • Preimplantation Genetic Testing  |  Aneuploidy detection of preimplantation embryos

Advancing Agricultural Genomics with Low-Cost NGS Genotyping


NEXTFLEX Small RNA-Seq with UDIs

Enhanced Data Security with UDIs to Propel your Small RNA Sequencing Applications

The NEXTFLEX® Unique Dual Index barcoded adapters (UDIs) for sequencing on Illumina® platforms provide enhanced data security by drastically increasing the multiplexing capacity while reducing costs by allowing users to pool multiple libraries onto a single flow cell lane, all while mitigating index hopping. The NEXTFLEX® small RNA-seq kit v4 incorporates a new dimension of data quality, integrity, and accuracy to offer a complete gel-free solution that is automated on the PerkinElmer liquid handlers. The UDI barcoded primers allow users to confidently increase their multiplexing capacity on the Illumina® MiSeq® HiSeq® 2000/2500, and NovaSeq® platforms for small RNA sequencing applications.

Rapid DNA-Seq XP

Automated DNA Library Prep for Sequencing on Illumina® Platforms

The NEXTFLEX® rapid XP DNA-seq kit combines enzymatic fragmentation with end-repair and A-tailing in one reaction to create a highly efficient first step in library generation. Resulting DNA-seq libraries show consistent library size, high yield, low GC-bias, and high coverage. All required reagents for fragmentation, library prep, and magnetic bead-based cleanup is included in the kit, which should be bundled with the NEXTFLEX® barcode of choice. This DNA-seq library prep kit accommodates a range of  sample inputs from 1 ng to 1 µg to generate high-quality libraries for Illumina® sequencers. Pre-plated reagents for automation are now available with single-use Unique Dual Index barcodes (UDIs) for an even simpler adoption experience on the PerkinElmer workstations.

RHS Technology: DOPlify® Kit

Start your Preimplantation Genetic Testing with the Right DNA Amplification

Genomic analysis platforms require at least 1 ng of DNA and single cells contain only 6 pg of genomic DNA. Whole genome amplification is used to replicate the complete genome to generate sufficient DNA for analysis. The accurate interpretation of single cell data relies on the ability of the whole genome amplification to comprehensively and reliably amplify the genome. The DOPlify® kit has been optimized specifically for single cell whole genome amplification. The kit is suited not just to single cells but to any low template sample, including cell free DNA and small numbers of cells.

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For research use only. Not for use in diagnostic procedures.